Kent M, Chandler R, Wachtel S
Center for Reproductive Biology, Spring Creek Ranch, Collierville, TN 38017.
Cytogenet Cell Genet. 1988;47(1-2):88-9. doi: 10.1159/000132514.
Accurate quantification of DNA from cells of several species is possible with flow cytometry. When one species is used as a reference, cytometric readings from two or more different species can be compared to obtain relative percent DNA or DNA indices. Differences in DNA from the male and female of the same species also can be measured. The method allows rapid screening of chromosomal abnormalities among large clinical populations, and evaluation of errors of sex determination such as XY sex reversal.
使用流式细胞术可以准确量化几种物种细胞中的DNA。当将一个物种用作参考时,可以比较来自两个或更多不同物种的细胞计数读数,以获得相对DNA百分比或DNA指数。同一物种的雄性和雌性之间的DNA差异也可以测量。该方法允许在大量临床人群中快速筛查染色体异常,并评估性别确定错误,如XY性反转。