Kotabova Eva, Malych Ronald, Pierella Karlusich Juan José, Kazamia Elena, Eichner Meri, Mach Jan, Lesuisse Emmanuel, Bowler Chris, Prášil Ondřej, Sutak Robert
Institute of Microbiology, Academy of Sciences, Centrum Algatech, Trebon, Czech Republic.
Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.
mSystems. 2021 Feb 9;6(1):e00738-20. doi: 10.1128/mSystems.00738-20.
The productivity of the ocean is largely dependent on iron availability, and marine phytoplankton have evolved sophisticated mechanisms to cope with chronically low iron levels in vast regions of the open ocean. By analyzing the metabarcoding data generated from the Oceans expedition, we determined how the global distribution of the model marine chlorarachniophyte varies across regions with different iron concentrations. We performed a comprehensive proteomics analysis of the molecular mechanisms underpinning the adaptation of to iron scarcity and report on the temporal response of cells to iron enrichment. Our results highlight the role of phytotransferrin in iron homeostasis and indicate the involvement of CREG1 protein in the response to iron availability. Analysis of the Oceans metagenomes and metatranscriptomes also points to a similar role for CREG1, which is found to be widely distributed among marine plankton but to show a strong bias in gene and transcript abundance toward iron-deficient regions. Our analyses allowed us to define a new subfamily of the CobW domain-containing COG0523 putative metal chaperones which are involved in iron metabolism and are restricted to only a few phytoplankton lineages in addition to At the physiological level, we elucidated the mechanisms allowing a fast recovery of PSII photochemistry after resupply of iron. Collectively, our study demonstrates that is well adapted to dynamically respond to a changing iron environment and suggests that CREG1 and COG0523 are important components of iron homeostasis in and other phytoplankton. Despite low iron availability in the ocean, marine phytoplankton require considerable amounts of iron for their growth and proliferation. While there is a constantly growing knowledge of iron uptake and its role in the cellular processes of the most abundant marine photosynthetic groups, there are still largely overlooked branches of the eukaryotic tree of life, such as the chlorarachniophytes. In the present work, we focused on the model chlorarachniophyte , integrating physiological and proteomic analyses in culture conditions with the mining of omics data generated by the Oceans expedition. We provide unique insight into the complex responses of to iron availability, including novel links to iron metabolism conserved in other phytoplankton lineages.
海洋的生产力在很大程度上取决于铁的可利用性，并且海洋浮游植物已经进化出复杂的机制来应对广阔公海中长期存在的低铁水平。通过分析“海洋”探险队产生的宏条形码数据，我们确定了模式海洋绿藻虫在不同铁浓度区域的全球分布情况。我们对支撑其适应铁缺乏的分子机制进行了全面的蛋白质组学分析，并报告了细胞对铁富集的时间响应。我们的结果突出了植物转铁蛋白在铁稳态中的作用，并表明CREG1蛋白参与了对铁可利用性的响应。对“海洋”宏基因组和宏转录组的分析也指出了CREG1的类似作用，发现它在海洋浮游生物中广泛分布，但在基因和转录本丰度上对缺铁区域有强烈偏向。我们的分析使我们能够定义一个新的含CobW结构域的COG0523假定金属伴侣亚家族，其参与铁代谢，并且除了[此处原文缺失相关内容]之外仅局限于少数浮游植物谱系。在生理水平上，我们阐明了铁重新供应后PSII光化学快速恢复的机制。总体而言，我们的研究表明[此处原文缺失相关内容]能够很好地动态响应不断变化的铁环境，并表明CREG1和COG0523是[此处原文缺失相关内容]和其他浮游植物中铁稳态的重要组成部分。尽管海洋中铁的可利用性较低，但海洋浮游植物的生长和繁殖仍需要大量的铁。虽然人们对铁的摄取及其在最丰富的海洋光合群体细胞过程中的作用的了解不断增加，但真核生物生命树中仍有很大一部分被忽视，例如绿藻虫。在本研究中，我们专注于模式绿藻虫，将培养条件下的生理和蛋白质组学分析与“海洋”探险队产生的组学数据挖掘相结合。我们对[此处原文缺失相关内容]对铁可利用性的复杂反应提供了独特的见解，包括与其他浮游植物谱系中保守的铁代谢的新联系。
