Jin Qi-Guan, Shi Wen-Ting, Wang Yu-Chen, Li Shu-Yan, Xue Chen, Xu Hao-Ran, Wu Mei-Tong, Wei Ying
College of Physical Education, Yangzhou University, Yangzhou, China.
Beijing Engineering Research Center of Protein and Functional Peptides, China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing, China.
Andrologia. 2021 May;53(4):e14005. doi: 10.1111/and.14005. Epub 2021 Feb 10.
This study evaluates the protective role of oyster peptide (OP) on the occurrence of Exercise-Hypogonadal Male Condition. Male rats were given heavy-load swimming training and / or OP was supplemented for 6 consecutive weeks. After heavy-load training, sperm count, sperm viability and sperm motility in epididymis, testosterone in serum and testis, glutathione peroxidase (GSH-px) and androgen receptor (AR) in testis and mating times were remarkably decreased, malondialdehyde (MDA), capture latency and mating latency were significantly increased, mRNA expression of steroidogenic acute regulatory (StAR) and P450 cholesterol side-chain cleavage enzyme (P450scc) were obviously down-regulated, but serum follicle-stimulating hormone (FSH) and luteinising hormone (LH) were not statistically changed. Conversely, when OP was supplemented at heavy-load training, sperm count, sperm viability and sperm motility in epididymis, serum FSH, LH, testosterone, GSH-px, superoxide dismutase (SOD), testosterone, AR in testis and mating times were dramatically increased, while testicular MDA, capture latency and mating latency were significantly decreased, and mRNA expression of StAR, StARD7, P450scc and 3β-hydroxysteroid dehydrogenase (3β-HSD) were significantly up-regulated. In conclusion, heavy-load training causes testicular spermatogenic and steroidogenic disorders by enhancing the generation of reactive oxygen species (ROS), which can be protected by the co-administration of OP by enhancing the function of pituitary gonad axis and lowering ROS generation.
本研究评估了牡蛎肽（OP）对运动性性腺功能减退男性状况发生的保护作用。雄性大鼠进行连续6周的负重游泳训练和/或补充OP。负重训练后，附睾中的精子数量、精子活力和精子运动能力、血清及睾丸中的睾酮、睾丸中的谷胱甘肽过氧化物酶（GSH-px）和雄激素受体（AR）以及交配次数均显著降低，丙二醛（MDA）、捕捉潜伏期和交配潜伏期显著增加，类固醇生成急性调节蛋白（StAR）和P450胆固醇侧链裂解酶（P450scc）的mRNA表达明显下调，但血清促卵泡生成素（FSH）和促黄体生成素（LH）无统计学变化。相反，在负重训练时补充OP，附睾中的精子数量、精子活力和精子运动能力、血清FSH、LH、睾酮、GSH-px、超氧化物歧化酶（SOD）、睾丸中的睾酮、AR以及交配次数均显著增加，而睾丸MDA、捕捉潜伏期和交配潜伏期显著降低，StAR、StARD7、P450scc和3β-羟基类固醇脱氢酶（3β-HSD）的mRNA表达显著上调。总之，负重训练通过增强活性氧（ROS）的生成导致睾丸生精和类固醇生成紊乱，而补充OP可通过增强垂体性腺轴功能和降低ROS生成来起到保护作用。
