ICAR - Indian Institute of Spices Research, Kozhikode, India.
ICAR - National Research Centre for Grapes, Pune, India.
J Biomol Struct Dyn. 2022 Sep;40(14):6398-6404. doi: 10.1080/07391102.2021.1883113. Epub 2021 Feb 10.
Rotundone, an oxygenated sesquiterpene compound, responsible for the peppery aroma. The importance of the rotundone in the flavor industry warrants search for the precursor genes in plants. We report in this study, the first on the identification of rotundone backbone genes viz., α-guaiene synthase & α-guaiene oxidase in black pepper. We identified the precursor genes of rotundone using berry transcriptome profiling. The metabolite profiling using head space mass spectrometry showed the presence of the direct precursor compounds for rotundone biosynthesis in black pepper berries. The identification of the genes & compounds of the guaiene skeleton is expected to help in bioprospecting of black pepper varieties & also in recombinant production of the aroma compound.Communicated by Ramaswamy H. Sarma [Formula: see text]Identification of rotundone backbone genes & precursor compounds from .
轮烯，一种含氧倍半萜化合物，负责产生辛辣香气。轮烯在香料行业的重要性使得人们有必要在植物中寻找其前体基因。我们在这项研究中报告了第一个关于在黑胡椒中鉴定轮烯骨干基因（即α-愈创木烯合酶和α-愈创木烯氧化酶）的情况。我们使用浆果转录组分析来鉴定轮烯的前体基因。使用顶空质谱法进行代谢产物分析表明，黑胡椒浆果中存在轮烯生物合成的直接前体化合物。轮烯骨架基因和化合物的鉴定有望有助于黑胡椒品种的生物勘探，以及香气化合物的重组生产。[化 1]由 Ramaswamy H. Sarma 通讯
