Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Methods Enzymol. 2020;645:119-140. doi: 10.1016/bs.mie.2020.07.005. Epub 2020 Aug 5.
The liquid biopsy preserves a noninvasive technique to analyze promising biomarkers in cell-free bodyfluids, mainly in cell-free plasma. The most cells secrete extracellular vesicles into the extracellular place which can be isolated, analyzed easily due to the wide range of different protocols and commercial kits. The mitochondrial DNA isolated from biofluids can serve as new view in early diagnosis of various diseases (e.g. cancers, cardiovascular diseases). In this chapter, possible protocols of mitochondrial DNA copy number quantification are discussed presenting some ways to determine the mtDNA level of extracellular vesicles in different diseases.
液体活检保留了一种非侵入性技术，用于分析无细胞体液中的有前途的生物标志物，主要是无细胞血浆中的生物标志物。大多数细胞会将细胞外囊泡分泌到细胞外，由于有广泛的不同方案和商业试剂盒，这些囊泡很容易被分离和分析。从生物流体中分离出的线粒体 DNA 可以作为各种疾病（例如癌症、心血管疾病）早期诊断的新视角。在本章中，讨论了线粒体 DNA 拷贝数定量的可能方案，介绍了在不同疾病中确定细胞外囊泡 mtDNA 水平的一些方法。
