Education and Research Center of Animal Models for Human Diseases, Fujita Health University, Toyoake, Aichi, Japan.
NIKON CeLL innovation Co., Ltd., Tokyo, Japan.
Methods Enzymol. 2020;645:231-242. doi: 10.1016/bs.mie.2020.09.006. Epub 2020 Oct 1.
Extracellular vesicles (EVs) are mediators of intercellular communication in diverse cellular functions. Visualizing EVs in vivo is important to elucidate the biogenesis of EVs, and various approaches have been developed for in vivo EV tracking. The ubiquitously expressed tetraspanin CD63 is liberally incorporated into EVs. Thus, fluorescently tagged CD63 has been used in many studies to label EVs. In the present study, we presented isolation and transfer assays for EVs from two transgenic rats expressing CD63-GFP in their body fluids or brains.
细胞外囊泡 (EVs) 是多种细胞功能中细胞间通讯的介质。在体内可视化 EVs 对于阐明 EVs 的生物发生非常重要,已经开发了各种方法用于体内 EV 追踪。广泛表达的四跨膜蛋白 CD63 大量包含在 EVs 中。因此,荧光标记的 CD63 已在许多研究中用于标记 EVs。在本研究中,我们展示了从两种在体液或大脑中表达 CD63-GFP 的转基因大鼠中分离和转移 EVs 的方法。