Simultaneous determination of atracurium besylate and its major decomposition products and related impurities by reversed-phase high-performance liquid chromatography.

The separation and determination of atracurium besylate and its major decomposition products and related impurities using octadecylsilica columns and acetonitrile-phosphate buffer mobile phases were studied. The influence of the acetonitrile and buffer concentrations and the pH of the mobile phase on the retention was investigated. The results indicated that hydrophobic and silanophilic interactions contribute to the retention of the compounds investigated.