Asada-Kubota M
Department of Anatomy, Kansai Medical University, Osaka, Japan.
J Histochem Cytochem. 1988 May;36(5):515-21. doi: 10.1177/36.5.3356895.
A monoclonal antibody (EPI-1), raised against the supernatant of a homogenate of Xenopus laevis larvae at the tailbud stage (stage 36/37), interacts specifically with a 250 KD epidermal antigen of Xenopus. An immunocytochemical gold-labeling technique was used to investigate changes in antigen distribution during epidermal development of Xenopus laevis. Specific immunolabeling was initially detected over the endoplasmic reticulum in the outer epithelial cells of the late gastrula stage (stage 12.5). After the early neurula stage (stage 13), immunolabeling appeared over moderately electron-dense bodies (these bodies disappear after stage 29), and also over the apical cell surface and adjacent cytoplasm of all the outer epithelial cells. During metamorphosis, labeling decreased and disappeared after stage 62, as the superficial layer had peeled off. These data suggest that the antigen is useful as a marker of general differentiation in studies of epidermal development during the embryonic and larval stages of Xenopus laevis.
一种单克隆抗体（EPI-1），是针对非洲爪蟾尾芽期（第36/37阶段）幼虫匀浆的上清液产生的，它与非洲爪蟾的一种250 KD表皮抗原特异性相互作用。采用免疫细胞化学金标记技术研究了非洲爪蟾表皮发育过程中抗原分布的变化。在原肠胚晚期（第12.5阶段）的外胚层细胞内质网上最初检测到特异性免疫标记。在神经胚早期（第13阶段）之后，免疫标记出现在中等电子密度的小体上（这些小体在第29阶段后消失），也出现在所有外胚层细胞的顶端细胞表面和相邻细胞质上。在变态过程中，随着表层脱落，标记在第62阶段后减少并消失。这些数据表明，该抗原在非洲爪蟾胚胎和幼虫阶段表皮发育研究中可作为一般分化的标志物。
