在扩散小室中用抗原包被的聚合物颗粒对放射性标记抗体进行体内评估。

In vivo evaluation of radiolabelled antibodies with antigen-coated polymer particles in diffusion chambers.

作者信息

Fjeld J G, Benestad H B, Stigbrand T, Nustad K

机构信息

Central Laboratory, Norwegian Radium Hospital, Oslo.

出版信息

J Immunol Methods. 1988 Apr 22;109(1):1-7. doi: 10.1016/0022-1759(88)90435-8.

DOI:10.1016/0022-1759(88)90435-8

PMID:3356905

Abstract

A method for the evaluation of in vivo immunolocalization of labelled monoclonal antibodies (MoAb) is presented. The technique is an alternative to the nude mouse xenograft system. The antigen reservoir is an intraperitoneal diffusion chamber (DC) filled with a suspension of antigen-coated polymer particles. Intravenously injected 125I-labelled MoAb are allowed to specifically bind to this artificial abdominal 'tumour', which can be removed and measured for radioactivity after animal killing. The model can be used as a preclinical in vivo method for the evaluation of labelled MoAbs prepared for immunodiagnostics or therapy. The DC system permits the amounts of both antibody and antigen to be controlled and antibody access to the antigen within the DC is presumably the same in every animal. The model permits a systematic comparison of different antibodies and antibody fragments, labels and labelling procedures, as well as routes of administration in immunocompetent animals.

摘要

本文介绍了一种评估标记单克隆抗体（MoAb）体内免疫定位的方法。该技术是裸鼠异种移植系统的替代方法。抗原储存器是一个腹腔内扩散室（DC），其中充满了抗原包被的聚合物颗粒悬浮液。静脉注射的125I标记的单克隆抗体可特异性结合到这个人工腹部“肿瘤”上，动物处死之后，该“肿瘤”可被移除并测量放射性。该模型可用作临床前体内方法，用于评估为免疫诊断或治疗制备的标记单克隆抗体。DC系统允许控制抗体和抗原的量，并且DC内抗体与抗原的接触在每只动物中可能是相同的。该模型允许对不同抗体和抗体片段、标记物和标记程序以及在免疫活性动物中的给药途径进行系统比较。