Zhou Liuting, Li Jianjuan, Pokhrel Ganga Raj, Chen Jun, Zhao Yanlin, Bai Ying, Zhang Chen, Lin Wenxiong, Wu Zeyan, Wu Chengzhen
Fujian Agriculture and Forestry University, Fuzhou, China.
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Plant Sci. 2021 Jan 25;11:578812. doi: 10.3389/fpls.2020.578812. eCollection 2020.
The growth and productivity of is negatively impacted by planting sickness under long-term monoculture regimes. In this study, Illumina MiSeq sequencing targeting genes was used to assess variations in the rhizospheric soil diazotrophic community under long-term monoculture rotations. Principal component analysis and unweighted pair-group method with arithmetic means (UPGMA) clustering demonstrated distinct differences in diazotrophic community structure between uncultivated soil (CK), the first rotation plantation (FCP), the second rotation plantation (SCP), and the third rotation plantation (TCP). Taxonomic analysis showed that the phyla increased while decreased under the consecutive monoculture (SCP and TCP). The relative abundance of , , , , , and increased significantly while , , and declined significantly at the genus level under consecutive monoculture (SCP and TCP). Redundancy analysis (RDA) showed that , and were positively correlated with total nitrogen and available nitrogen. In conclusion, continuous monoculture could change the structure of diazotrophic microbes in the rhizosphere, resulting in the imbalance of the diazotrophic bacteria population, which might be a crucial factor related to replanting disease in this cultivated tree species.
在长期单一栽培模式下，种植病害会对[具体作物名称未给出]的生长和生产力产生负面影响。在本研究中，使用靶向[具体基因名称未给出]基因的Illumina MiSeq测序来评估长期单一栽培轮作下根际土壤固氮微生物群落的变化。主成分分析和算术平均非加权组对法（UPGMA）聚类表明，未开垦土壤（CK）、第一轮作种植园（FCP）、第二轮作种植园（SCP）和第三轮作种植园（TCP）之间的固氮微生物群落结构存在明显差异。分类学分析表明，在连续单一栽培（SCP和TCP）下，[具体门名称未给出]门增加而[具体门名称未给出]门减少。在连续单一栽培（SCP和TCP）下，[具体属名称未给出]属、[具体属名称未给出]属、[具体属名称未给出]属、[具体属名称未给出]属、[具体属名称未给出]属和[具体属名称未给出]属的相对丰度显著增加，而[具体属名称未给出]属、[具体属名称未给出]属和[具体属名称未给出]属显著下降。冗余分析（RDA）表明，[具体因素未给出]与总氮和有效氮呈正相关。总之，连续[具体作物名称未给出]单一栽培会改变根际固氮微生物的结构，导致固氮细菌种群失衡，这可能是该栽培树种连作障碍的一个关键因素。
