温度会影响鼠单克隆IgG抗体与蛋白A的结合。
Temperature affects binding of murine monoclonal IgG antibodies to protein A.
作者信息
Tu Y Y, Primus F J, Goldenberg D M
机构信息
Center for Molecular Medicine and Immunology, University of Medicine and Dentistry of New Jersey, Newark 07103.
出版信息
J Immunol Methods. 1988 Apr 22;109(1):43-7. doi: 10.1016/0022-1759(88)90440-1.
Murine IgG subclasses show different affinities when they react with protein A. The temperature of application of the sample to protein A-Sepharose markedly influences the interaction of certain antibodies with this specific adsorbent. Elution of weakly bound mouse IgG could be carried out by mild temperature changes instead of lowering the pH, and this should be advantageous with certain immunoglobulins that are labile under acidic conditions.
小鼠IgG亚类与蛋白A反应时表现出不同的亲和力。将样品应用于蛋白A-琼脂糖的温度显著影响某些抗体与这种特异性吸附剂的相互作用。弱结合的小鼠IgG可以通过温和的温度变化而不是降低pH值来洗脱，这对于某些在酸性条件下不稳定的免疫球蛋白应该是有利的。
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