Laboratoire Central de l'Elevage (LABOCEL), Niamey, Niger.
Laboratoire National de l'Elevage et de Recherches Vétérinaires (LNERV), Dakar, Senegal.
Vet Med Sci. 2021 May;7(3):979-986. doi: 10.1002/vms3.439. Epub 2021 Feb 11.
Mycoplasma mycoides subsp. mycoides (Mmm) is the aetiological agent of contagious bovine pleuropneumonia (CBPP). The aim of the present study was to identify the profiles of the Mmm strains isolated in Niger using the 'Multilocus Sequence Analysis' (MLSA) typing technique based on polymorphism analysis of housekeeping and non-coding genes. The investigation was conducted on samples (n=22) comprising of lung tissues, lymph node and pleural fluids. Following classical PCR, Mmm positive amplicons (n=6) were identified. These positive amplicons were then amplified using eight loci of the PG1 reference strain (LocPG1-0001, Loc-PG1-0103, Loc-PG1-0287, Loc-PG1-0431, Loc-PG1-0489, Loc-PG1-0523, Loc-PG1-0710 and Loc-PG1-0827). Sequencing followed by the determination of the profile of each strain by the combination of the allele numbers revealed three different MLSA profiles namely; A11, E01 and A15. The profiles A11 and E01 were previously identified. The novel profile identified in this study was named profile A15. The difference was detected while comparing sequences of non-coding loci. This novel profile was named 'A15' according to the similarities with African reference strain profile 'A00' at the seven loci level (loc-0103, loc-0287, loc-0431, loc-0489, loc-0523, loc-0710 and loc-0827). For CBPP control measures, identification and molecular characterization of Mmm strains is very important. Thus, the use of MLSA technique is relevant to identify profiles of Mmm circulating in Niger. Other countries where CBPP is still endemic are encouraged to use a MLSA scheme to address this issue and, most importantly, to rapidly trace back the origin of outbreaks, which will help reduce the transmission and spread of the disease. In addition, mapping the profiles of strains circulating in each of the countries of the sub-region is necessary for effective control of CBPP.
绵羊无乳支原体亚种（Mmm）是传染性牛胸膜肺炎（CBPP）的病原体。本研究的目的是使用基于管家基因和非编码基因多态性分析的“多位点序列分析”（MLSA）分型技术，鉴定尼日尔分离的 Mmm 株的特征。该调查使用了 22 个肺组织、淋巴结和胸腔液样本（n=22）。在进行经典 PCR 后，鉴定出 Mmm 阳性扩增子（n=6）。然后使用 PG1 参考株的 8 个基因座（LocPG1-0001、Loc-PG1-0103、Loc-PG1-0287、Loc-PG1-0431、Loc-PG1-0489、Loc-PG1-0523、Loc-PG1-0710 和 Loc-PG1-0827）对这些阳性扩增子进行扩增。测序后，通过组合等位基因数量确定每个菌株的特征，发现了三种不同的 MLSA 特征，分别为 A11、E01 和 A15。A11 和 E01 特征先前已被鉴定。本研究中鉴定的新型特征命名为 A15 特征。通过比较非编码基因座的序列检测到差异。根据与非洲参考株在七个基因座水平（loc-0103、loc-0287、loc-0431、loc-0489、loc-0523、loc-0710 和 loc-0827）上的相似性，将这种新型特征命名为“A15”。对于 CBPP 控制措施，鉴定和分子特征分析 Mmm 菌株非常重要。因此，使用 MLSA 技术是识别尼日尔流行的 Mmm 株特征的有效方法。其他仍有 CBPP 流行的国家鼓励使用 MLSA 方案来解决这一问题，最重要的是，迅速追踪疫情的来源，这将有助于减少疾病的传播和蔓延。此外，为了有效控制 CBPP，有必要绘制该次区域各国流行的菌株特征图谱。
