School of Clinical Medicine Research Laboratory, Nelson R Mandela School of Medicine, 56394University of KwaZulu-Natal, Durban, South Africa.
Department of Obstetrics and Gynaecology, School of Clinical Medicine, Nelson R Mandela School of Medicine, 56394University of KwaZulu-Natal, Durban, South Africa.
Int J STD AIDS. 2021 Apr;32(5):396-402. doi: 10.1177/0956462420971439. Epub 2021 Feb 11.
The detection of using culture assays is challenging. This study aims to compare different assays for the detection of . This cross-sectional study was conducted at King Edward VIII Hospital and included 307 antenatal attendees, each willing to provide two endocervical swabs. The first swab was used for culture identification of , and the second swab was processed for the detection of the pathogen by the TaqMan quantitative polymerase chain reaction (qPCR) assay, an in-house () PCR and PCR detection of the gene. Culture and the nucleic acid amplification assays were each used as comparator tests in the analysis. Sensitivity and specificity were calculated using RS Studio. The prevalence of was 7.8%. When compared to the TaqMan assay, the PCR exhibited the highest sensitivity of 62%, with a substantial level of agreement (kappa level of agreement: 0.60), followed by the PCR (38%) with a moderate level of agreement (0.52) and culture exhibiting the lowest sensitivity of 25% with a fair level of agreement (0.38). The diagnostic accuracy of all the assays was >90%. The TaqMan qPCR assay has the ability to serve as a future diagnostic assay for the detection of .
使用培养检测法检测 具有挑战性。本研究旨在比较不同的检测方法用于检测 。本横断面研究在爱德华八世国王医院进行，纳入了 307 名产前就诊者，每位就诊者均愿意提供两个宫颈内拭子。第一个拭子用于培养鉴定 ，第二个拭子用于通过 TaqMan 定量聚合酶链反应 (qPCR) 检测、内部 () PCR 和 基因 PCR 检测来检测病原体。在分析中，将培养和核酸扩增检测法均用作比较检测法。使用 RS Studio 计算敏感性和特异性。 的患病率为 7.8%。与 TaqMan 检测法相比， PCR 的敏感性最高，为 62%，具有较高的一致性（kappa 一致性水平：0.60），其次是 PCR（38%），具有中等水平的一致性（0.52），而培养的敏感性最低，为 25%，一致性为中等水平（0.38）。所有检测法的诊断准确性均>90%。TaqMan qPCR 检测法具有作为未来检测 的诊断检测法的潜力。
