Yoo J U, Kresina T F, Malemud C J, Goldberg V M
Department of Orthopaedics, University Hospitals of Cleveland, OH.
J Orthop Res. 1988;6(3):389-96. doi: 10.1002/jor.1100060310.
This study details the macromolecular changes in cartilage involving proteoglycan molecules in an animal model of rheumatoid arthritis. In experimental chronic immune synovitis, fluorescein-conjugated mouse IgG and three monoclonal antibodies (MAbs 2G2, 2E9, and 6C9) portraying differing fine antigenic specificity for rabbit cartilage proteoglycan monomer were utilized to detail alterations in cartilage proteoglycan. In normal and IgG-immune animals, fluorescein isothiocynate (FITC)-conjugated MAbs 2G2 and 2E9 stained cellular/pericellular (C/PC) region intensely. FITC-MAb 2G2 stained cartilage interterritorial matrix as well. FITC-MAb 6C9 stained only C/PC area lightly but did not stain matrix. A marked decrease in staining intensity with FITC-MAb 2G2 was noted in cartilage sections derived from animals with immune synovitis. A corresponding increase in staining of cartilage was noted with FITC-MAb 6C9. The augmented staining of articular cartilage with FITC-MAb 6C9 was most prominent in femoral condyle tissue sections, which corresponded to the cartilaginous area, with the greatest severity in gross pathology. There was a slight augmentation of staining with FITC-MAb 2E9, especially in the C/PC area of medial/femoral cartilage. In addition, the animals with immune synovitis showed abortive cartilage repair exemplified by the presence of chondrocyte cloning (up to 20 cells) which correlated with increased FITC-MAb 2G2 staining. The differential MAb staining patterns of cartilaginous tissues obtained utilizing FITC-conjugated monoclonal antibodies with known fine antigenic specificity indicates a modulation of proteoglycans involving predominantly core protein epitopes in the articular cartilage of animals with chronic immune synovitis.
本研究详细阐述了类风湿性关节炎动物模型中软骨内涉及蛋白聚糖分子的大分子变化。在实验性慢性免疫性滑膜炎中，使用了与荧光素结合的小鼠IgG以及三种对兔软骨蛋白聚糖单体具有不同精细抗原特异性的单克隆抗体（单克隆抗体2G2、2E9和6C9）来详细描述软骨蛋白聚糖的变化。在正常动物和接受IgG免疫的动物中，异硫氰酸荧光素（FITC）结合的单克隆抗体2G2和2E9强烈染色细胞/细胞周围（C/PC）区域。FITC-单克隆抗体2G2也对软骨的 territorial 间基质进行染色。FITC-单克隆抗体6C9仅轻度染色C/PC区域，但不染色基质。在患有免疫性滑膜炎的动物的软骨切片中，观察到FITC-单克隆抗体2G2的染色强度显著降低。同时观察到FITC-单克隆抗体6C9对软骨的染色相应增加。FITC-单克隆抗体6C9对关节软骨的增强染色在股骨髁组织切片中最为明显，该区域对应于软骨区域，在大体病理学中病变最严重。FITC-单克隆抗体2E9的染色略有增强，尤其是在内侧/股骨软骨的C/PC区域。此外，患有免疫性滑膜炎的动物表现出软骨修复失败，表现为存在软骨细胞克隆（多达20个细胞），这与FITC-单克隆抗体2G2染色增加相关。利用具有已知精细抗原特异性的FITC结合单克隆抗体获得的软骨组织的不同单克隆抗体染色模式表明，在患有慢性免疫性滑膜炎的动物的关节软骨中，蛋白聚糖的调节主要涉及核心蛋白表位。
