State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 710032, Xi'an, China; State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Fourth Military Medical University, 710032, Xi'an, China.
Department of Experiment Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, China.
Biochem Biophys Res Commun. 2021 Mar 26;546:65-73. doi: 10.1016/j.bbrc.2021.02.001. Epub 2021 Feb 8.
The occurrence and development of tumors cannot be separated from the influence of differentiation at different stages and levels. Our study found that E-cadherin was significantly increased in cell model induced by sodium butyrate and cell density, while METTL3, METTL16 and WTAP were decreased during the differentiation of cells. In the clinicopathological tissues, E-cadherin was low expressed in poorly differentiated tumor tissues and above three regulators were highly expressed in poorly differentiated tissues. At the levels of clinicopathological differentiation, tissue differentiation and cell differentiation, the result indicated that the poor prognosis of colorectal cancer (CRC) may be closely related to high expression of total m6A level and high expression of METTL3, METTL16 and WTAP.
肿瘤的发生和发展离不开不同阶段和水平的分化影响。我们的研究发现，丁酸钠诱导细胞模型和细胞密度时，E-钙黏蛋白显著增加，而 METTL3、METTL16 和 WTAP 在细胞分化过程中减少。在临床病理组织中，低分化肿瘤组织中 E-钙黏蛋白表达降低，以上三种调节因子在低分化组织中高表达。在临床病理分化、组织分化和细胞分化水平上，结果表明结直肠癌（CRC）预后不良可能与总 m6A 水平升高和 METTL3、METTL16 和 WTAP 高表达密切相关。
