感染性逆转录病毒RNA的体外合成。
In vitro synthesis of infectious retroviral RNA.
作者信息
Flamant F, Sorge J A
机构信息
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037.
出版信息
J Virol. 1988 May;62(5):1827-31. doi: 10.1128/JVI.62.5.1827-1831.1988.
Abstract
A plasmid was constructed in which a T7 RNA polymerase promoter was placed upstream of a recombinant amphotropic retrovirus genome containing a selectable neomycin resistance gene. To test the infectivity of the RNA produced by T7 RNA polymerase in vitro, the RNA was microinjected into the nuclei of psi 2 packaging cells. Infectious particles conferring G418 resistance were released.
摘要
构建了一种质粒，其中T7 RNA聚合酶启动子位于含有可选择的新霉素抗性基因的重组嗜双嗜性逆转录病毒基因组的上游。为了测试T7 RNA聚合酶在体外产生的RNA的感染性，将该RNA显微注射到psi 2包装细胞的细胞核中。释放出赋予G418抗性的感染性颗粒。
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