Ausejo Raquel, Martínez Juan Manuel, Soler-Llorens Pedro, Bolarín Alfonso, Tejedor Teresa, Falceto María Victoria
Department of Animal Pathology, Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zaragoza, 50013 Zaragoza, Spain.
Department of Biotechnology R&D, Magapor S.L., 50600 Ejea de los Caballeros, Spain.
Animals (Basel). 2021 Feb 9;11(2):465. doi: 10.3390/ani11020465.
There are numerous cases when conventional spermiogram parameters are all within an acceptable range but boar subfertility persists. The total sperm nuclear DNA fragmentation index (tDFI) is a trait related to fertility and prolificacy problems that is not routinely evaluated in commercial AI boars. The aim of this research was to study the effect of the photoperiod, season and reproductive age of the boar on tDFI (measured by SCSA) of 1279 ejaculates from 372 different boars belonging to 6 different breeds located in 6 AI studs in Spain. tDFI data ranged from 0.018% to 20.1%. Although there was a significant single boar effect in the tDFI occurrence, a negative correlation between the tDFI and the age of the boar was found ( < 0.001). tDFI would decrease due to aging of the boar 0.66% each year old within the observed age range. After including age as a covariate in the ANCOVA, no differences were found in tDFI between photoperiods when the sperm collection date was evaluated. However, when the date of the production of semen in the testis was evaluated, the total percentage of spermatozoa with fragmented nuclear DNA was 1.46% higher in the increasing photoperiod in comparison to the decreasing photoperiod ( < 0.0001). On the other hand, for both dates, the lowest tDFI values corresponded to minimum day length for decreasing photoperiod phase (autumn), while the highest tDFI values were found in summer (maximum day length for decreasing photoperiod phase).
在许多情况下，常规精子活力参数均在可接受范围内，但公猪的生育力低下问题依然存在。精子总核DNA碎片化指数（tDFI）是一个与生育力和繁殖力问题相关的指标，在商业人工授精公猪中通常不进行常规评估。本研究的目的是研究光照周期、季节和公猪繁殖年龄对来自西班牙6个不同人工授精站的6个不同品种的372头公猪的1279份精液的tDFI（通过精子染色质结构分析测量）的影响。tDFI数据范围为0.018%至20.1%。虽然tDFI的出现存在显著的个体公猪效应，但发现tDFI与公猪年龄之间存在负相关（<0.001）。在观察到的年龄范围内，公猪每衰老一岁，tDFI会下降0.66%。在协方差分析中纳入年龄作为协变量后，在评估采精日期时，不同光照周期下的tDFI没有差异。然而，在评估睾丸中精液产生日期时，与光照周期缩短相比，光照周期增加时核DNA碎片化的精子总百分比高1.46%（<0.0001）。另一方面，对于这两个日期，最低的tDFI值对应于光照周期缩短阶段（秋季）的最短日长，而最高的tDFI值出现在夏季（光照周期缩短阶段的最长日长）。
