Liu Da, Walcott Ronald, Mis Solval Kevin, Chen Jinru
Department of Food Science and Technology, The University of Georgia, Griffin, GA 30602, USA.
Department of Plant Pathology, The University of Georgia, Athens, GA 30602, USA.
Foods. 2021 Jan 31;10(2):285. doi: 10.3390/foods10020285.
Interests in using biological agents for control of human pathogens on vegetable seeds are rising. This study evaluated whether probiotic bacterium GG, bacterial strains previously used as biocontrol agents in plant science, as well as a selected plant pathogen could compete with foodborne human pathogens, such as and enterohemorrhagic (EHEC), for growth in microbiological media and attachment to vegetable seeds; and to determine whether the metabolites in cell-free supernatants of competitive bacterial spent cultures could inhibit the growth of the two pathogens. The results suggest that the co-presence of competitive bacteria, especially GG, significantly ( < 0.05) inhibited the growth of and EHEC. Cell-free supernatants of GG cultures significantly reduced the pathogen populations in microbiological media. Although not as effective as GG in inhibiting the growth of and EHEC, the biocontrol agents were more effective in competing for attachment to vegetable seeds. The study observed the inhibition of human bacterial pathogens by competitive bacteria or their metabolites and the competitive attachment to sprout seeds among all bacteria involved. The results will help strategize interventions to produce vegetable seeds and seed sprouts free of foodborne pathogens.
利用生物制剂控制蔬菜种子上的人类病原体的兴趣正在增加。本研究评估了益生菌GG、先前在植物科学中用作生物防治剂的细菌菌株以及一种选定的植物病原体是否能够与食源性人类病原体（如[此处原文缺失具体病原体名称]和肠出血性大肠杆菌（EHEC））竞争，以在微生物培养基中生长并附着于蔬菜种子；并确定竞争性细菌用过的培养物的无细胞上清液中的代谢产物是否能抑制这两种病原体的生长。结果表明，竞争性细菌（尤其是GG）的共同存在显著（P<0.05）抑制了[此处原文缺失具体病原体名称]和EHEC的生长。GG培养物的无细胞上清液显著减少了微生物培养基中的病原体数量。虽然在抑制[此处原文缺失具体病原体名称]和EHEC的生长方面不如GG有效，但生物防治剂在竞争附着于蔬菜种子方面更有效。该研究观察到竞争性细菌或其代谢产物对人类细菌病原体的抑制作用以及所有参与细菌之间对发芽种子的竞争性附着。这些结果将有助于制定干预策略，以生产不含食源性病原体的蔬菜种子和豆芽。
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