Kruk Marcin, Trząskowska Monika
Faculty of Human Nutrition, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, 02-776 Warsaw, Poland.
Food Hygiene and Quality Management, Department of Food Gastronomy and Food Hygiene, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, 02-776 Warsaw, Poland.
Foods. 2021 Mar 5;10(3):542. doi: 10.3390/foods10030542.
This study aimed to analyse the impact of sanitation methods on the formation of bacterial biofilms after disinfection and during the germination process of mung bean on seeds and in the germination environment. Moreover, the influence of 299v on the growth of the tested pathogenic bacteria was evaluated. Three strains of and were used for the study. The colony forming units (CFU), the crystal violet (CV), the LIVE/DEAD and the gram fluorescent staining, the light and the scanning electron microscopy (SEM) methods were used. The tested microorganisms survive in a small number. During germination after disinfection D2 (20 min HO at 60 °C, then 15 min in a disinfecting mixture consisting of HO, HO and CH₃COOH), the biofilms grew most after day 2, but with the DP2 method (D2 + 299v during germination) after the fourth day. Depending on the method used, the second or fourth day could be a time for the introduction of an additional growth-limiting factor. Moreover, despite the use of seed disinfection, their germination environment could be favourable for the development of bacteria and, consequently, the formation of biofilms. The appropriate combination of seed disinfection methods and growth inhibition methods at the germination stage will lead to the complete elimination of the development of unwanted microflora and their biofilms.
本研究旨在分析卫生方法对绿豆种子消毒后及发芽过程中，以及发芽环境中细菌生物膜形成的影响。此外，还评估了299v对受试病原菌生长的影响。研究使用了三种菌株。采用菌落形成单位（CFU）、结晶紫（CV）、活/死和革兰氏荧光染色、光学和扫描电子显微镜（SEM）方法。受试微生物少量存活。在消毒D2（60℃下20分钟过氧化氢处理，然后在由过氧化氢、过氧化氢和醋酸组成的消毒混合物中处理15分钟）后的发芽过程中，生物膜在第2天后生长最多，但采用DP2方法（发芽期间D2 + 299v）则在第4天后。根据所使用的方法，第二天或第四天可能是引入额外生长限制因素的时间。此外，尽管对种子进行了消毒，但其发芽环境可能有利于细菌生长，进而导致生物膜形成。种子消毒方法和发芽阶段生长抑制方法的适当组合将导致完全消除有害微生物及其生物膜的生长。
