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黄瓜花叶病毒运动蛋白的 28 个氨基酸在症状形成和胞间连丝定位中起作用。

The 28 Ser Amino Acid of Cucumber Mosaic Virus Movement Protein Has a Role in Symptom Formation and Plasmodesmata Localization.

机构信息

Centre for Agricultural Research, Plant Protection Institute, Herman Ottó Street 15., H-1022 Budapest, Hungary.

Department of Plant Pathology, Faculty of Horticultural Science, Szent István University, Villányi Street 29-43., H-1118 Budapest, Hungary.

出版信息

Viruses. 2021 Jan 31;13(2):222. doi: 10.3390/v13020222.

DOI:10.3390/v13020222
PMID:33572676
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7912182/
Abstract

Cucumber mosaic virus (CMV, , Bromoviridae) is an economically significant virus infecting important horticultural and field crops. Current knowledge regarding the specific functions of its movement protein (MP) is still incomplete. In the present study, potential post-translational modification sites of its MP were assayed with mutant viruses: MP/S28A, MP/S28D, MP/S120A and MP/S120D. Ser28 was identified as an important factor in viral pathogenicity on cv. Xanthi, and . The subcellular localization of GFP-tagged movement proteins was determined with confocal laser-scanning microscopy. The wild type movement protein fused to green fluorescent protein (GFP) (MP-eGFP) greatly colocalized with callose at plasmodesmata, while MP/S28A-eGFP and MP/S28D-eGFP were detected as punctate spots along the cell membrane without callose colocalization. These results underline the importance of phosphorylatable amino acids in symptom formation and provide data regarding the essential factors for plasmodesmata localization of CMV MP.

摘要

黄瓜花叶病毒（CMV，Bromoviridae）是一种经济上重要的病毒，感染重要的园艺作物和大田作物。目前对于其移动蛋白（MP）的特定功能的了解仍然不完全。在本研究中，通过突变病毒对其 MP 的潜在翻译后修饰位点进行了检测：MP/S28A、MP/S28D、MP/S120A 和 MP/S120D。Ser28 被确定为在 cv. Xanthi 和 上病毒致病性的重要因素。利用共焦激光扫描显微镜确定了 GFP 标记的运动蛋白的亚细胞定位。与绿色荧光蛋白（GFP）融合的野生型运动蛋白（MP-eGFP）与胞间连丝处的胼胝质强烈共定位，而 MP/S28A-eGFP 和 MP/S28D-eGFP 则被检测为沿细胞膜的点状斑点，没有胼胝质共定位。这些结果强调了可磷酸化氨基酸在症状形成中的重要性，并提供了关于 CMV MP 质膜定位的必需因素的数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/d9300c38a213/viruses-13-00222-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/2aeebe19400b/viruses-13-00222-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/c0afc2ff661c/viruses-13-00222-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/79336414d3ec/viruses-13-00222-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/81299b943ca2/viruses-13-00222-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/e326a2270a7b/viruses-13-00222-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/d9300c38a213/viruses-13-00222-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/2aeebe19400b/viruses-13-00222-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/c0afc2ff661c/viruses-13-00222-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/79336414d3ec/viruses-13-00222-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/81299b943ca2/viruses-13-00222-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/e326a2270a7b/viruses-13-00222-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/7912182/d9300c38a213/viruses-13-00222-g006.jpg

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