Schepens Eye Research Institute of Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
New England Eye Center/Department of Ophthalmology, Tufts University Medical School, Boston, MA 02111, USA.
Cells. 2021 Jan 30;10(2):274. doi: 10.3390/cells10020274.
Chemokines are an extended group of chemoattractant cytokines responsible for the recruitment of leukocytes into tissues. Among them, interferon-γ-inducible protein 10 (CXCL10) is abundantly expressed following inflammatory stimuli and participates in the trafficking of monocytes and activated T cells into sites of injury. Here, we report that different members of the galectin family of carbohydrate-binding proteins promote the expression and synthesis of CXCL10 independently of interferon-γ. Interestingly, CXCL10 induction was observed when galectins came in contact with stromal fibroblasts isolated from human cornea but not other cell types such as epithelial, monocytic or endothelial cells. Induction of CXCL10 by the tandem repeat galectin-8 was primarily associated with the chemotactic migration of THP-1 monocytic cells, whereas the prototype galectin-1 promoted the CXCL10-dependent migration of Jurkat T cells. These results highlight the potential importance of the galectin signature in dictating the recruitment of specific leukocyte populations into precise tissue locations.
趋化因子是一组趋化因子细胞因子，负责将白细胞募集到组织中。其中，干扰素-γ诱导蛋白 10（CXCL10）在炎症刺激后大量表达，并参与单核细胞和活化 T 细胞向损伤部位的迁移。在这里，我们报告说，糖结合蛋白家族的不同成员的半乳糖凝集素独立于干扰素-γ促进 CXCL10 的表达和合成。有趣的是，当半乳糖凝集素与从人角膜分离的基质成纤维细胞接触时，观察到 CXCL10 的诱导，但其他细胞类型如上皮细胞、单核细胞或内皮细胞则没有。串联重复半乳糖凝集素-8 诱导 CXCL10 的主要与 THP-1 单核细胞的趋化迁移相关，而原型半乳糖凝集素-1 则促进 Jurkat T 细胞依赖 CXCL10 的迁移。这些结果强调了半乳糖凝集素特征在决定特定白细胞群募集到特定组织位置中的潜在重要性。
