雌三醇与血浆蛋白的结合

Oestriol binding to plasma proteins.

作者信息

Moutsatsou V, Oakey R E

机构信息

Department of Chemical Pathology, School of Medicine, University of Leeds, England.

出版信息

J Steroid Biochem. 1988 Mar;29(3):319-23. doi: 10.1016/0022-4731(88)90033-7.

DOI:10.1016/0022-4731(88)90033-7

PMID:3357338

Abstract

Simple diffusion experiments indicated that oestriol was retained by human pregnancy plasma more effectively than by albumin solutions of a corresponding concentration. Oestriol bound (Ka = 6 X 10(6) l/mol at 4 degrees C) to a glycoprotein which had been isolated from plasma by adsorption to Concanavalin A. The free energy of binding at 37 degrees C was -38 kJ/mol. Competition experiments indicated that the oestriol binding glycoprotein had properties expected of sex hormone binding globulin. The distribution of oestriol among the protein fractions of human pregnancy plasma--glycoprotein bound 7.8%, albumin bound 78.6%, unbound 13.6%--suggests that this glycoprotein plays little part in the transport of oestriol.

摘要

简单扩散实验表明，雌三醇在人妊娠血浆中的保留效果比在相应浓度的白蛋白溶液中更有效。雌三醇在4℃时与通过伴刀豆球蛋白A吸附从血浆中分离出的一种糖蛋白结合（Ka = 6×10⁶ l/mol）。37℃时的结合自由能为-38 kJ/mol。竞争实验表明，雌三醇结合糖蛋白具有性激素结合球蛋白所预期的特性。人妊娠血浆蛋白组分中雌三醇的分布情况——糖蛋白结合7.8%，白蛋白结合78.6%，未结合13.6%——表明这种糖蛋白在雌三醇的转运中作用不大。