Establishment and Application of a Dual TaqMan Real-Time PCR Method for and .

species are common opportunistic bacteria and foodborne pathogens. The proper detection of can effectively reduce the occurrence of food-borne public health events. and are the two most important pathogens in the genus. In this study, a dual TaqMan Real-Time PCR method was established to simultaneously detect and distinguish and in samples. The method exhibited good specificity, stability, and sensitivity. Specifically, the minimum detection concentrations of and in pure bacterial cultures were 6.08 × 10 colony forming units (CFU)/ml and 4.46 × 10 CFU/ml, respectively. Additionally, the minimum detectable number of and in meat and milk was 10 CFU/g. In addition, the method can be used to distinguish between strains of and within two hours. Overall, it is a sensitive, easy-to-use, and practical test for the identification and classification of in food.