Shurygina Irina A, Shurygin Мichael G, Rodionova Lubov V, Ayushinova Nataliya I
Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia.
Pharmasyntez, Irkutsk, Russia.
Pleura Peritoneum. 2020 Nov 2;5(4):20200114. doi: 10.1515/pp-2020-0114. eCollection 2020 Nov.
To study the expression of growth factors in the regulation of tissue repair after peritoneal damage tissue response to peritoneal damage.
Experimental study in 35 male Wistar rats determining the evolution over time of the tissue response to aseptic peritoneal damage. A standardized bowel and peritoneal lesions were created in the right lower quadrant by laparotomy. Then, tissular expression of growth factors was evaluated by multiplex polymerase chain reaction at seven timepoints between 6 h and 30 days, postoperatively.
Tissular responses of granulocyte-stimulating factors (Csf2, Csf3), connective tissue growth factor (Ctgf), epidermal growth factors and receptor (Egf, Egfr), fibroblast growth factors (Fgf2, 7 and 10), heparin binding EGF-like growth factor (Hbegf), hepatocyte growth factor (Hgf), insulin-like growth factor-1 (Igf1), mitogenic transforming growth factors (Tgfa, Tgfb1, Tgfbr3), and vascular endothelial growth factor A (Vegfa) were biphasic with a first expression peak at day 3, followed by a more pronounced peak at day 14.
We observed a long-lasting, widespread response of tissular growth factors for at least two weeks after peritoneal damage. To be clinically effective, the prophylaxis of postoperative adhesions might be needed for an extended period of time.
研究生长因子在腹膜损伤后组织修复调节及组织对腹膜损伤反应中的表达。
对35只雄性Wistar大鼠进行实验研究，确定无菌性腹膜损伤后组织反应随时间的演变。通过剖腹术在右下腹制造标准化的肠和腹膜损伤。然后，在术后6小时至30天的7个时间点，通过多重聚合酶链反应评估生长因子的组织表达。
粒细胞刺激因子（Csf2、Csf3）、结缔组织生长因子（Ctgf）、表皮生长因子及其受体（Egf、Egfr）、成纤维细胞生长因子（Fgf2、7和10）、肝素结合表皮生长因子样生长因子（Hbegf）、肝细胞生长因子（Hgf）、胰岛素样生长因子-1（Igf1）、促有丝分裂转化生长因子（Tgfa、Tgfb1、Tgfbr3）和血管内皮生长因子A（Vegfa）的组织反应呈双相性，在第3天出现第一个表达峰值，随后在第14天出现更明显的峰值。
我们观察到腹膜损伤后至少两周内组织生长因子存在持久、广泛的反应。为达到临床效果，可能需要长时间预防术后粘连。
