Medical College of Qinghai University, Xining, Qinghai 810016, China 2Qinghai University, Xining, Qinghai 810016, China.
J Biosci. 2021;46.
The protective effect of astrocytes on nerves was demonstrated by mitochondrial transfer. The neuroprotective effect of hypoxic pretreatment is widely accepted. The aim of this research is to investigate the role of hypoxic preconditioning on astrocytes mitochondria. Rat neuronal cells and astrocytes were isolated and cultured. A hypoxic preconditioned astrocyte and oxygen glucose deprivation (OGD) neuronal cell co-culture experiment was used to detect the effect of hypoxic preconditioning (HP) on nerve damage. The silencing of proliferatoractivated receptor γ coactivator-1α (PGC-1α) with siRNA was used to explore the role of HP in the repair of nerve damage and biogenesis of mitochondria. HP increased astrocyte viability and promoted neuroprotective factor secretion. The expression levels of antioxidant enzymes, PGC-1α and uncoupling protein2 (UCP2) were up-regulated by HP. In addition, HP improved mitochondrial function and reduced oxidative stress induced by OGD. It was found that HP astrocytes had a greater neuroprotective effect than normal astrocytes cells. Neuronal apoptosis and reactive oxygen species levels were down-regulated by cell co-culture. The PGC-1α siRNA experiment showed that hypoxia treatment promotes mitochondrial biogenesis and plays a neuroprotective role. HP significantly enhanced the efficacy of astrocytes in the treatment of neuronal injury.
星形胶质细胞通过线粒体转移对神经起到保护作用。低氧预处理的神经保护作用已得到广泛认可。本研究旨在探讨低氧预处理对星形胶质细胞线粒体的作用。分离并培养大鼠神经元细胞和星形胶质细胞。通过低氧预处理星形胶质细胞和氧葡萄糖剥夺（OGD）神经元细胞共培养实验，检测低氧预处理（HP）对神经损伤的影响。用 siRNA 沉默增殖激活受体 γ 共激活因子-1α（PGC-1α），探讨 HP 在神经损伤修复和线粒体生物发生中的作用。HP 增加星形胶质细胞活力并促进神经营养因子分泌。HP 上调抗氧化酶、PGC-1α 和解偶联蛋白 2（UCP2）的表达水平。此外，HP 改善了线粒体功能并减轻了 OGD 引起的氧化应激。结果发现，HP 星形胶质细胞比正常星形胶质细胞具有更强的神经保护作用。细胞共培养可下调神经元凋亡和活性氧水平。PGC-1α siRNA 实验表明，低氧处理促进线粒体生物发生并发挥神经保护作用。HP 显著增强了星形胶质细胞治疗神经元损伤的效果。
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