Zhao Yuqi, Fu Qingjie, Cui Xiaoqian, Chi Hui, Lu Yongzhuang, Liu Xiaoli, Yu Miao, Fei Qiang, Feng Guodong, Shan Hongyan, Huan Yanfu
College of Chemistry, Jilin University, Changchun 130023, People's Republic of China.
Department of Emergency and Critical Care, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China.
Anal Methods. 2021 Feb 28;13(8):1069-1074. doi: 10.1039/d0ay02283g. Epub 2021 Feb 12.
In this study, gold-platinum nanoparticles (Au@PtNPs) with peroxidase-like activity were synthesized. In the absence of thiourea (TU), the Au@PtNPs can catalyze the decomposition of hydrogen peroxide, and oxidize 3,3',5,5'-tetramethylbenzidine dihydrochloride (TMB, colorless) into oxidized 3,3',5,5'-tetramethylbenzidine dihydrochloride (oxTMB, blue). The peroxidase-like activity of the Au@PtNPs is inhibited in the presence of TU, and TMB cannot be oxidized to oxTMB effectively, and no blue color could be observed. Based on this finding, a novel colorimetric sensor for detecting TU is proposed. The absorbance response curve showed a good linearity for the concentration of TU in the range of 10 nmol L to 10 μmol L with a correlation coefficient of R = 0.999, and the limit of detection is 9.57 nmol L. The colorimetric sensor possesses excellent selectivity, anti-interference ability, and application value in actual samples.
在本研究中，合成了具有类过氧化物酶活性的金铂纳米颗粒（Au@PtNPs）。在不存在硫脲（TU）的情况下，Au@PtNPs可催化过氧化氢分解，并将3,3',5,5'-四甲基联苯胺二盐酸盐（TMB，无色）氧化为氧化型3,3',5,5'-四甲基联苯胺二盐酸盐（oxTMB，蓝色）。在TU存在下，Au@PtNPs的类过氧化物酶活性受到抑制，TMB无法有效氧化为oxTMB，且观察不到蓝色。基于这一发现，提出了一种用于检测TU的新型比色传感器。吸光度响应曲线在10 nmol/L至10 μmol/L的TU浓度范围内呈现良好的线性关系，相关系数R = 0.999，检测限为9.57 nmol/L。该比色传感器具有优异的选择性、抗干扰能力以及在实际样品中的应用价值。
