Department of Biosystems Engineering, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Department of Stomatology, Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, P.R. China.
Mol Med Rep. 2021 Apr;23(4). doi: 10.3892/mmr.2021.11903. Epub 2021 Feb 12.
(Dunn) Nakai, commonly known as gondre, is a perennial herb that grows predominantly in South Korea. It contains several bioactive phytochemicals with antioxidant, anti‑cancer, anti‑tumor and anti‑inflammatory properties. The present study aimed to investigate the effects of methanolic extracts of gondre on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). As characterized by nuclear magnetic resonance spectroscopy and matrix‑assisted laser deposition/ionization (time‑of‑flight) mass spectrometry, the methanol extract of gondre was found to be enriched with pectolinarin. After 48 h, enhanced viability of hPDLSCs was observed in the presence of gondre compared with under control conditions, suggesting the biocompatibility of gondre. Notably, biocompatibility was markedly affected by gondre concentration in cultured media. Relatively high cell viability was observed in medium containing 0.05% gondre. Furthermore, mineralization was significantly higher in hPDLSCs in the presence of gondre compared with that in control cells, indicating their mineralization potential. Increased expression of various transcription markers, such as collagen 1, runt‑related transcription factor 2, bone sialoprotein and alkaline phosphatase, was also detected when hPDLSCs were stimulated with gondre compared with in the control groups, further confirming the superior osteogenic potential of gondre extract for tissue engineering applications, particularly in bone tissues.
(Dunn)Nakai，俗称贯叶金丝桃，是一种多年生草本植物，主要生长在韩国。它含有几种具有抗氧化、抗癌、抗肿瘤和抗炎特性的生物活性植物化学物质。本研究旨在探讨贯叶金丝桃甲醇提取物对人牙周膜干细胞(hPDLSCs)成骨分化的影响。核磁共振波谱和基质辅助激光解吸/电离(飞行时间)质谱分析表明，贯叶金丝桃甲醇提取物富含圣草酚。与对照条件相比，贯叶金丝桃存在时 hPDLSCs 的活力增强，提示贯叶金丝桃具有生物相容性。值得注意的是，生物相容性明显受到培养基中贯叶金丝桃浓度的影响。在含有 0.05%贯叶金丝桃的培养基中观察到相对较高的细胞活力。此外，与对照细胞相比，在贯叶金丝桃存在的情况下，hPDLSCs 的矿化明显更高，表明其矿化潜力。与对照组相比，当 hPDLSCs 受到贯叶金丝桃刺激时，各种转录标志物如胶原蛋白 1、 runt 相关转录因子 2、骨涎蛋白和碱性磷酸酶的表达也增加，进一步证实了贯叶金丝桃提取物在组织工程应用，特别是在骨组织中的优越成骨潜力。
