系统发生学赋予功能：MAR-FISH。

Assigning Function to Phylogeny: MAR-FISH.

机构信息

Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.

出版信息

Methods Mol Biol. 2021;2246:225-236. doi: 10.1007/978-1-0716-1115-9_14.

Abstract

Microautoradiography (MAR) is a technique by which assimilated radioactive tracers incorporated into the biomass can be detected by a film emulsion. This allows for the testing of cellular preferences in electron donors and acceptors of individual cells in complex microbial assemblages, as well as the ability to take up substrates under diverse environmental exposures.Combination with staining techniques such as fluorescence in situ hybridization (FISH) can be used to identify the involved cells. Here, the practical aspects of a combined microautoradiography and fluorescence in situ hybridization (MAR-FISH) approach are described.

摘要

显微放射自显影（MAR）是一种技术，可以通过胶片乳剂检测到掺入生物量中的放射性示踪剂。这允许测试复杂微生物组合中单个细胞的电子供体和受体对电子供体和受体的细胞偏好，以及在不同环境暴露下摄取底物的能力。与荧光原位杂交（FISH）等染色技术结合使用，可以鉴定涉及的细胞。本文描述了一种结合显微放射自显影和荧光原位杂交（MAR-FISH）方法的实际应用。