UKRAINIAN MEDICALSTOMATOLOGICAL ACADEMY,POLTAVA, UKRAINE.
Wiad Lek. 2020;73(12 cz 1):2612-2616.
The aim: Thepaper wasaimedat thestudyof thebiomineralization processesofa permanentdental crown in thepostnatal period of histogenesis.
Materials and methods: The study involved 30 culled puppies aged 30-40 days. To study the histogenesis of the germs of the permanent tooth from the cuticular epithelium in the postnatal period microscopic, electron microscopic, immunohistochemical methods of study have been used.
Results: The studies show that in the postnatal period, the maturation of the germ of a permanent tooth starts with the synthesis of cells of the cuticular epithelium of the organic stroma, capable of subsequent mineralization. Differentiation of the proameloblasts, located on the surface of the dental papillary mesenchyma, at the early stages of histogenesis, is strongly associated with the appearance of a specific protein taftelin. Origination of secretory ameloblasts, which produce the protein enamelin, triggers the process of secondary biomineralization of the enamel. The terminal processes of the secretory ameloblasts produce the protein in the form of layers that overlap each other at a certain angle. Such layering of the structures of enamel and dentin contributes to the S-shaped maturation of the hard tooth tissues, strengthening them considerably. At the follicle stage, maturation of the dental crown, coated with cuticular epithelium, occurs. Invaginations of the cuticular epithelium form a characteristic topography of the dental crown, and enamel projections are further formed by the ameloblasts. Epitheliocytes of the inner layer of the enamel organ have desmosomal connections that allow the filtration of the salivary fluid at the stage of the enamel trophism.
Conclusions: The findings of the study suggest that, normally, due to the cuticular epithelium, filtration of the salivary fluid occurs with protein deposition on it and subsequent infiltration of the calcium salts into the subjacent enamel.
本文旨在研究牙釉质在组织发生的出生后阶段的生物矿化过程。
材料和方法：本研究涉及 30 只 30-40 天大的被淘汰的小狗。为了研究出生后切皮上皮向永久性牙齿芽的组织发生，使用了显微镜、电子显微镜、免疫组织化学研究方法。
研究表明，在出生后时期，永久性牙齿芽的成熟始于有机基质的表皮细胞的合成，这些细胞能够随后矿化。位于牙乳头间质表面的前成釉细胞的分化，在组织发生的早期阶段，与特定蛋白质塔夫林的出现密切相关。分泌性成釉细胞的起源，其产生蛋白釉原蛋白，引发牙釉质的二次生物矿化过程。分泌性成釉细胞的终末突起以相互重叠一定角度的层状形式产生该蛋白。这种牙釉质和牙本质结构的分层有助于硬组织牙齿的 S 形成熟，使其强度大大提高。在滤泡阶段，覆盖有表皮的牙冠成熟。表皮的内陷形成牙冠的特征拓扑结构，进一步由成釉细胞形成牙釉质突起。牙釉质器官内层的上皮细胞具有桥粒连接，允许在牙釉质营养阶段过滤唾液。
研究结果表明，正常情况下，由于表皮，唾液发生过滤，蛋白质在其上沉积，并随后将钙盐渗透到相邻的牙釉质中。
