在酸性条件下，由 d(AGA)形成的稳定的未完成四聚体 G-四链体。
A stable uncompleted tetramolecular G-quadruplex formed by d(AGA) under acidic condition.
机构信息
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China; Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China.
出版信息
Int J Biol Macromol. 2021 Apr 15;176:66-71. doi: 10.1016/j.ijbiomac.2021.02.059. Epub 2021 Feb 9.
In this work, the effects of terminal adenines on the formation and stability of tetramolecular G-quadruplexes (G4s) have been studied by electrospray ionization mass spectrometry (ESI-MS), UV, CD and NMR spectroscopy. Several evidences suggested that the sequences d(AGA) (n = 4 or 5) form stable uncompleted tetramolecular G4 at acidic condition which is different from the canonical one in the neutral condition. In addition, hydrolysis of guanine has also been observed in acidic condition that may occur for unpaired strands rather than in complete G4. Thus, a new G4 topology containing incomplete G-quartet is proposed that is very stable and particularly presents in acidic ammonium ions solution. The information presented in this study provides the new insight on the polymorphism of G4s in acidic environment, which may help understand of the special role of adenines on the formation of G4s.
在这项工作中，通过电喷雾质谱（ESI-MS）、紫外、圆二色性（CD）和核磁共振（NMR）光谱研究了末端腺嘌呤对四聚体 G-四链体（G4）形成和稳定性的影响。有几个证据表明，序列 d(AGA)（n = 4 或 5）在酸性条件下形成稳定的未完成四聚体 G4，这与中性条件下的典型 G4 不同。此外，在酸性条件下也观察到鸟嘌呤的水解，这可能发生在未配对的链上，而不是在完整的 G4 中。因此，提出了一种含有不完全 G-四联体的新型 G4 拓扑结构，该结构非常稳定，特别是在酸性铵离子溶液中存在。本研究提供的信息为酸性环境中 G4 的多态性提供了新的认识，这可能有助于理解腺嘌呤在 G4 形成中的特殊作用。
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