Institute of Quality Standard & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing 100081, China.
Institute of Quality Standard & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing 100081, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Mar 15;1167:122564. doi: 10.1016/j.jchromb.2021.122564. Epub 2021 Jan 26.
The aim of this work is to contribute to the assessment of multi-residue analysis of veterinarian and human pharmaceuticals using UHPLC-QTOF in livestock urine and blood (cattle, chicken, sheep and pig). Firstly, an in-house database including compound name, monoisotopic mass, chemical formula, retention time, chemical structure, and three CID MS-MS spectra of the 234 selected drugs were built for qualitative detection. Secondly, the method validation result showed that all the 234 drugs exhibited good linearity with determination coefficients (R) higher than 0.999. Then, the distribution of the drugs recoveries, intra-day RSD and inter-day RSD results for all seven matrices were tested. Finally, after a carefully cross check, 150 veterinarian and human pharmaceuticals could meet the methodological requirements (recovery, 50-120%; intra-day RSD ≤ 15%, inter-day RSD ≤ 20%) in all seven matrices. Our results suggested that although the main applications of UHPLC-QTOF are directed towards detection and identification of the compounds, this method should be also applied for quantitative purposes.
本工作旨在评估使用 UHPLC-QTOF 对牲畜尿液和血液（牛、鸡、羊和猪）中的兽医和人用药物进行多残留分析。首先，为定性检测构建了一个包含化合物名称、单一同位素质量、化学式、保留时间、化学结构和 234 种选定药物的三种 CID MS-MS 光谱的内部数据库。其次，方法验证结果表明，所有 234 种药物均表现出良好的线性关系，决定系数（R）均高于 0.999。然后，测试了所有七种基质中药物回收率、日内 RSD 和日间 RSD 结果的分布。最后，经过仔细的交叉检查，150 种兽医和人用药物在所有七种基质中均能满足方法要求（回收率为 50-120%；日内 RSD≤15%，日间 RSD≤20%）。我们的结果表明，尽管 UHPLC-QTOF 的主要应用是检测和鉴定化合物，但该方法也应适用于定量目的。
