Suat M P, Kek H C, Jin Ai A A T
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Trop Biomed. 2016 Sep 1;33(3):476-485.
Salmonella enterica is one of the leading causes of human foodborne infections. The objectives of this study are to investigate S. enterica prevalence in sushi and sashimi in Malaysia, to determine the presence of virulence genes and the antimicrobial resistance profiles of isolated S. enterica. In the 200 samples tested, 16% were positive for S. enterica. Sixty-six percent of the S. enterica isolates harboured at least one virulence gene and the most common virulence gene was sifA (37.5%). Antibiotic susceptibility testing showed 65.6% (21/32) of the isolates to be resistant to at least one antibiotic tested, with sulfamethoxazole resistance as the most common (50%). Resistance to the drugs-of-choice (fluoroquinolones and third-generation cephalosporin) for severe salmonellosis were also detected - ceftriaxone (25%), ceftazidime (28.1%) and ciprofloxacin (9.4%). Two isolates (9.5%) were resistant to all antibiotic tested while 12 isolates (37.5%) exhibited multi-drug resistance (MDR) with 10 different MDR profiles. Most of the isolates presented MDR profilesAP, AUG, FOX, NA (penicillins, beta-lactams, cephems and quinolone) with or without the addition of other drugs. In conclusion, the high rate of S. enterica prevalence in the sampled sushi and sashimi warrants increased safety measures for sushi and sashimi preparation.
肠炎沙门氏菌是人类食源性感染的主要病因之一。本研究的目的是调查马来西亚寿司和生鱼片中肠炎沙门氏菌的流行情况，确定分离出的肠炎沙门氏菌毒力基因的存在情况及其抗菌药物耐药谱。在检测的200份样本中，16%的样本肠炎沙门氏菌呈阳性。66%的肠炎沙门氏菌分离株携带至少一种毒力基因，最常见的毒力基因是sifA（37.5%）。药敏试验显示，65.6%（21/32）的分离株对至少一种测试抗生素耐药，其中对磺胺甲恶唑耐药最为常见（50%）。还检测到对严重沙门氏菌病的首选药物（氟喹诺酮类和第三代头孢菌素）的耐药情况——头孢曲松（25%）、头孢他啶（28.1%）和环丙沙星（9.4%）。有两株分离株（9.5%）对所有测试抗生素均耐药，而12株分离株（37.5%）表现出多重耐药（MDR），具有10种不同的MDR谱型。大多数分离株呈现MDR谱型AP、AUG、FOX、NA（青霉素类、β-内酰胺类、头孢菌素类和喹诺酮类），有或没有添加其他药物。总之，抽样的寿司和生鱼片中肠炎沙门氏菌的高流行率表明需要加强寿司和生鱼片制备过程中的安全措施。
