联合大分子生物材料和流体切应力促进马脂肪间充质干细胞的成骨分化能力。
Combined macromolecule biomaterials together with fluid shear stress promote the osteogenic differentiation capacity of equine adipose-derived mesenchymal stem cells.
机构信息
Institute of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University of Giessen, Frankfurter Str. 98, 35392, Giessen, Germany.
Clinic of Small Animals, c/o Institute of Veterinary Anatomy, Histology and Embryology, Justus Liebig University of Giessen, 35392, Giessen, Germany.
出版信息
Stem Cell Res Ther. 2021 Feb 12;12(1):116. doi: 10.1186/s13287-021-02146-7.
BACKGROUND
Combination of mesenchymal stem cells (MSCs) and biomaterials is a rapidly growing approach in regenerative medicine particularly for chronic degenerative disorders including osteoarthritis and osteoporosis. The present study examined the effect of biomaterial scaffolds on equine adipose-derived MSC morphology, viability, adherence, migration, and osteogenic differentiation.
METHODS
MSCs were cultivated in conjunction with collagen CultiSpher-S Microcarrier (MC), nanocomposite xerogels B30 and combined B30 with strontium (B30Str) biomaterials in osteogenic differentiation medium either under static or mechanical fluid shear stress (FSS) culture conditions. The data were generated by histological means, live cell imaging, cell viability, adherence and migration assays, semi-quantification of alkaline phosphatase (ALP) activity, and quantification of the osteogenic markers runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) expression.
RESULTS
The data revealed that combined mechanical FSS with MC but not B30 enhanced MSC viability and promoted their migration. Combined osteogenic medium with MC, B30, and B30Str increased ALP activity compared to cultivation in basal medium. Osteogenic induction with MC, B30, and B30Str resulted in diffused matrix mineralization. The combined osteogenic induction with biomaterials under mechanical FSS increased Runx2 protein expression either in comparison to those cells cultivated in BM or those cells induced under static culture. Runx2 and ALP expression was upregulated following combined osteogenic differentiation together with B30 and B30Str regardless of static or FSS culture.
CONCLUSIONS
Taken together, the data revealed that FSS in conjunction with biomaterials promoted osteogenic differentiation of MSCs. This combination may be considered as a marked improvement for clinical applications to cure bone defects.
背景
间充质干细胞（MSCs）与生物材料的组合是再生医学中一种快速发展的方法，特别是在治疗骨关节炎和骨质疏松症等慢性退行性疾病方面。本研究旨在探讨生物材料支架对马脂肪来源间充质干细胞形态、活力、黏附、迁移和成骨分化的影响。
方法
将 MSCs 与胶原 Cultispher-S 微载体（MC）、纳米复合水凝胶 B30 以及含锶的 B30（B30Str）生物材料在成骨分化培养基中共同培养，在静态或机械流体剪切力（FSS）培养条件下进行。通过组织学手段、活细胞成像、细胞活力、黏附和迁移试验、碱性磷酸酶（ALP）活性的半定量分析以及成骨标志物 runt 相关转录因子 2（Runx2）和碱性磷酸酶（ALP）表达的定量分析来生成数据。
结果
数据显示，与 B30 相比，机械 FSS 与 MC 联合使用可提高 MSC 的活力并促进其迁移。与基础培养基相比，MC、B30 和 B30Str 联合成骨培养基可提高 ALP 活性。MC、B30 和 B30Str 的成骨诱导导致基质矿化扩散。与在 BM 中培养的细胞或在静态培养下诱导的细胞相比，在机械 FSS 下与生物材料联合进行成骨诱导可增加 Runx2 蛋白表达。无论在静态或 FSS 培养下，与 B30 和 B30Str 联合进行成骨分化均可上调 Runx2 和 ALP 表达。
结论
总之，数据表明 FSS 与生物材料联合可促进 MSCs 的成骨分化。这种组合可能被认为是改善临床应用治疗骨缺损的显著进展。
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