Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, D-30625 Hannover, Germany.
Department of Cell Biology and Biophysics, Leibniz University Hannover, D-30419 Hannover, Germany.
Dent Mater. 2021 Mar;37(3):534-546. doi: 10.1016/j.dental.2020.12.008. Epub 2021 Feb 10.
The angiogenic differentiation of dental pulp stem cells (DPSCs) is important for tissue homeostasis and wound healing. In this study the influence of 2-hydroxyethyl methacrylate (HEMA) on angiogenic differentiation was investigated.
To evaluate HEMA effects on angiogenic differentiation, DPSCs were cultivated in angiogenic differentiation medium (ADM) in the presence or absence of non-toxic HEMA concentrations (0.1 mM and 0.5 mM). Subsequently, angiogenic differentiation was analyzed on the molecular level by qRT-PCR and protein profiler analyzes of angiogenic markers and flow cytometry of PECAM1. The influence of HEMA on angiogenic phenotypes was analyzed by cell migration and sprouting assays.
Treatment with 0.5 mM HEMA during differentiation can lead to a slight reduction of angiogenic markers on mRNA level. HEMA also seems to slightly reduce the quantity of angiogenic cytokines (not significant). However, these HEMA concentrations have no detectable influence on cell migration, the abundance of PECAM1 and the formation of capillaries. Higher concentrations caused primary cytotoxic effects in angiogenic differentiation experiments conducted for longer periods than 72 h.
Non-cytotoxic HEMA concentrations seem to have a minor impact on the expression of angiogenic markers, essentially on the mRNA level, without affecting the angiogenic differentiation process itself on a detectable level.
牙髓干细胞（DPSCs）的血管生成分化对于组织稳态和伤口愈合很重要。本研究探讨了 2-羟乙基甲基丙烯酸酯（HEMA）对血管生成分化的影响。
为了评估 HEMA 对血管生成分化的影响，将 DPSCs 在含有或不含有无毒 HEMA 浓度（0.1 mM 和 0.5 mM）的血管生成分化培养基（ADM）中培养。随后，通过 qRT-PCR 和血管生成标志物的蛋白谱分析以及 PECAM1 的流式细胞术，在分子水平上分析血管生成分化。通过细胞迁移和发芽测定分析 HEMA 对血管生成表型的影响。
在分化过程中用 0.5 mM HEMA 处理可导致血管生成标志物在 mRNA 水平上略有降低。HEMA 似乎也略微降低了血管生成细胞因子的数量（无统计学意义）。然而，这些 HEMA 浓度对细胞迁移、PECAM1 的丰度和毛细血管的形成没有可检测到的影响。在进行超过 72 小时的血管生成分化实验时，较高浓度会导致原发性细胞毒性作用。
非细胞毒性 HEMA 浓度似乎对血管生成标志物的表达有轻微影响，主要在 mRNA 水平上，而在可检测水平上对血管生成分化过程本身没有影响。
