Institut de biologie moléculaire des plantes, UPR2357 du CNRS, Université de Strasbourg, Strasbourg, France.
Institut Jacques Monod, Laboratory of Molecular Virology, UMR7592 CNRS, Université de Paris, Paris, France.
Nat Commun. 2021 Feb 12;12(1):1007. doi: 10.1038/s41467-021-21338-6.
Plant viruses cause massive crop yield loss worldwide. Most plant viruses are RNA viruses, many of which contain a functional tRNA-like structure. RNase P has the enzymatic activity to catalyze the 5' maturation of precursor tRNAs. It is also able to cleave tRNA-like structures. However, RNase P enzymes only accumulate in the nucleus, mitochondria, and chloroplasts rather than cytosol where virus replication takes place. Here, we report a biotechnology strategy based on the re-localization of plant protein-only RNase P to the cytosol (CytoRP) to target plant viruses tRNA-like structures and thus hamper virus replication. We demonstrate the cytosol localization of protein-only RNase P in Arabidopsis protoplasts. In addition, we provide in vitro evidences for CytoRP to cleave turnip yellow mosaic virus and oilseed rape mosaic virus. However, we observe varied in vivo results. The possible reasons have been discussed. Overall, the results provided here show the potential of using CytoRP for combating some plant viral diseases.
植物病毒导致全球农作物大量减产。大多数植物病毒是 RNA 病毒，其中许多含有具有功能的 tRNA 样结构。RNase P 具有催化前体 tRNA 5'成熟的酶活性。它还能够切割 tRNA 样结构。然而，RNase P 酶仅在细胞核、线粒体和叶绿体中积累，而不在病毒复制发生的细胞质中。在这里，我们报告了一种基于将植物蛋白仅 RNase P 重新定位到细胞质（CytoRP）以靶向植物病毒 tRNA 样结构从而阻碍病毒复制的生物技术策略。我们证明了在拟南芥原生质体中蛋白仅 RNase P 的细胞质定位。此外，我们提供了 CytoRP 体外切割芜菁黄花叶病毒和油菜 mosaic 病毒的证据。然而，我们观察到体内结果存在差异。已经讨论了可能的原因。总的来说，这里的结果表明使用 CytoRP 来对抗一些植物病毒病的潜力。
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