Department of Molecular Biology Test Technique, College of Medical Technology, Beihua University, Jilin, China.
Department of Pharmacology, College of Pharmacy, Beihua University, Jilin, China.
Biosci Biotechnol Biochem. 2021 Mar 24;85(4):834-841. doi: 10.1093/bbb/zbaa100.
Schisandrin B (Sch B), a lignan compound in Schisandra, possesses antioxidant, anti-inflammatory, and antiobesity activities. The effect of Sch B on melanogenesis and molecular mechanisms are still unknown. Therefore, we aimed to investigate the antimelanogenic effects of Sch B on α-melanocyte-stimulating hormone-induced B16F10 cells and elucidate the underlying molecular mechanisms. We found that Sch B significantly suppressed melanin content and mushroom tyrosinase (TYR) activity. Sch B treatment decreased the expression of TYR, melanocyte-inducing transcription factor (MITF), tyrosinase-related protein (TRP) 1, and TRP2. Moreover, Sch B modulated the phosphorylation of p38, extracellular-regulated protein kinase, c-Jun N-terminal kinase, and cAMP-response element binding protein (CREB), implying that these pathways may be involved in suppressing melanogenesis. Furthermore, we found that Sch B decreased melanogenesis by downregulating MITF and melanogenic enzymes via MAPK and CREB pathways. Overall, these findings indicate that Sch B has the potential use in whitening.
五味子素 B（Sch B）是五味子中的一种木脂素化合物，具有抗氧化、抗炎和抗肥胖活性。Sch B 对黑色素生成的影响及其分子机制尚不清楚。因此，我们旨在研究 Sch B 对 α-黑色素细胞刺激激素诱导的 B16F10 细胞的抗黑色素生成作用，并阐明其潜在的分子机制。我们发现 Sch B 可显著抑制黑色素含量和蘑菇酪氨酸酶（TYR）活性。Sch B 处理可降低 TYR、黑素细胞诱导转录因子（MITF）、酪氨酸酶相关蛋白（TRP）1 和 TRP2 的表达。此外，Sch B 调节丝裂原活化蛋白激酶（MAPK）和环磷酸腺苷反应元件结合蛋白（CREB）通路中 p38、细胞外调节蛋白激酶、c-Jun N-末端激酶的磷酸化，表明这些途径可能参与抑制黑色素生成。此外，我们发现 Sch B 通过下调 MAPK 和 CREB 通路中的 MITF 和黑色素生成酶来降低黑色素生成。总的来说，这些发现表明 Sch B 具有美白的潜力。
