Department of Laboratory Medicine, Hallym University College of Medicine, Dongtan Sacred Heart Hospital, Hwaseong-si, Republic of Korea.
Department of Laboratory Medicine, Hallym University College of Medicine, Hallym, University Sacred Heart Hospital, Anyang-si, Republic of Korea.
Transplant Proc. 2021 Jun;53(5):1762-1770. doi: 10.1016/j.transproceed.2021.01.011. Epub 2021 Feb 10.
Human preformed antibodies (Abs), anti-galactose-alpha-1,3-galactose (Gal) and anti-N-glycolylneuraminic acid (Neu5Gc), can react with porcine antigens of wild-type pigs. To provide basic population data of the Abs for potential application in clinical xenotransplantation, we developed enzyme-linked immunosorbent assay methods and investigated the serum titers of anti-Gal and anti-Neu5Gc Abs, including immunoglobulin (Ig) M and IgG along with its subclasses, in humans.
Anti-Gal and anti-Neu5Gc Abs serum titers were measured in 380 healthy Korean adults using the in-house enzyme-linked immunosorbent assays. The frequency and median values of anti-Gal and anti-Neu5Gc were measured, and their class and subclass distribution were evaluated.
The detection frequencies of anti-Gal were 99.2%, 95.0%, 23.2%, 94.5%, 12.4%, and 3.4% for IgM, IgG, IgG1, IgG2, IgG3, and IgG4, respectively. The detection frequencies of anti-Neu5Gc Abs were 87.4%, 96.6%, 1.6%, 46.3%, 0.0%, and 0.0% for IgM, IgG, IgG1, IgG2, IgG3, and IgG4, respectively. The median values of anti-Gal IgM (1001.6 ng/mL) and IgG (1198.3 ng/mL) were significantly higher than those of anti-Neu5Gc Abs (IgM, 328.4 ng/mL; IgG, 194.7 ng/mL; P < .001). IgG2 titers of both anti-Gal and anti-Neu5Gc Abs correlated better with the IgG class than the titers of other IgG subclasses.
The titers of anti-Gal Abs were higher than those of anti-Neu5Gc Abs. IgG2 was the main IgG subclass in both anti-Gal and anti-Neu5Gc Abs. Variation in the titers of anti-Gal or anti-Neu5Gc Abs may partly explain the biological and immunologic changes that occur in recipients of xenotransplants.
人体预先形成的抗体（Abs），抗半乳糖-α-1,3-半乳糖（Gal）和抗 N-糖酰基神经氨酸（Neu5Gc），可以与野生型猪的猪抗原发生反应。为了为临床异种移植提供潜在应用的 Abs 的基本人群数据，我们开发了酶联免疫吸附测定方法，并调查了人类抗 Gal 和抗 Neu5Gc Abs 的血清滴度，包括免疫球蛋白（Ig）M 和 IgG 及其亚类。
使用内部酶联免疫吸附测定法在 380 名健康韩国成年人中测量抗 Gal 和抗 Neu5Gc Abs 的血清滴度。测量抗 Gal 和抗 Neu5Gc 的频率和中位数，并评估其类别和亚类分布。
抗 Gal 的检测频率分别为 IgM、IgG、IgG1、IgG2、IgG3 和 IgG4 的 99.2%、95.0%、23.2%、94.5%、12.4%和 3.4%。抗 Neu5Gc Abs 的检测频率分别为 IgM、IgG、IgG1、IgG2、IgG3 和 IgG4 的 87.4%、96.6%、1.6%、46.3%、0.0%和 0.0%。抗 Gal IgM（1001.6 ng/mL）和 IgG（1198.3 ng/mL）的中位数明显高于抗 Neu5Gc Abs（IgM，328.4 ng/mL；IgG，194.7 ng/mL；P <.001）。抗 Gal 和抗 Neu5Gc Abs 的 IgG2 滴度与 IgG 类的相关性优于其他 IgG 亚类的滴度。
抗 Gal Abs 的滴度高于抗 Neu5Gc Abs。IgG2 是抗 Gal 和抗 Neu5Gc Abs 中的主要 IgG 亚类。抗 Gal 或抗 Neu5Gc Abs 滴度的变化可能部分解释了异种移植受者发生的生物学和免疫变化。
