Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
J Endod. 2021 May;47(5):793-799. doi: 10.1016/j.joen.2021.02.003. Epub 2021 Feb 11.
The purpose of this study was to assess the antimicrobial activity and flow of root canal sealers after incorporating novel highly loaded antimicrobial drug-silica coassembled particles (DSPs).
DSPs were synthesized through coassembly of silica and octenidine dihydrochloride (OCT) antimicrobial surfactant. DSPs were loaded (1% and 2% wt) into epoxy resin sealer (AH Plus [AH]; Dentsply DeTrey GmbH, Konstanz, Germany) or calcium silicate-based sealer (EndoSequence Bioceramic Sealer (BC); Brasseler, Savannah, GA). OCT release from DSP-modified sealers was determined using liquid chromatography. Antimicrobial activity of sealers against planktonic or biofilm form Enterococcus faecalis was assessed using direct contact and membrane restricted tests. Sealer flow was tested according to ISO6876:2012.
OCT release from BC + 1% or 2% DSPs was above the minimum inhibitory concentration following 2 days throughout the 30-day experiment, whereas OCT release from AH + 1% or 2% DSP was significantly below the minimum inhibitory concentration against E. faecalis (4 μg/mL) over the whole 30-day experimental period. All materials (with or without DSPs) killed planktonic bacteria initially. AH ± 1% or 2% DSPs had no antimicrobial activity after 7 days. BC + 1% or 2% DSPs maintained antibacterial activity over the 30-day period. Both modified and unmodified sealers completely inhibited the growth of E. faecalis biofilms after 24 hours of contact. DSPs decreased the flow of AH and BC sealers; for AH, the reduction was proportional to the amount of DSPs added. All modified and unmodified sealers, except for AH + 2% DSPs, were within the acceptable limits of ISO 6876 flow tests.
DSPs enhanced the antimicrobial performance of BC but not AH, whereas the material's flow remained compliant with ISO 6876 standards. Depending on the sealer, DSPs may enhance antimicrobial efficacy in root canal treatment and potentially improve treatment outcome.
本研究旨在评估新型高载抗菌药物-硅共组装颗粒（DSPs）掺入根管封闭剂后的抗菌活性和流动性能。
通过硅和盐酸奥替尼啶（OCT）抗菌表面活性剂的共组装合成 DSPs。将 DSPs（1%和 2%wt）负载到环氧树脂封闭剂（AH Plus [AH]；Dentsply DeTrey GmbH，康斯坦茨，德国）或硅酸钙基封闭剂（EndoSequence 生物陶瓷封闭剂（BC）；Brasseler，萨凡纳，GA）中。使用液相色谱法测定 DSP 改性封闭剂中 OCT 的释放情况。使用直接接触和膜限制试验评估封闭剂对浮游或生物膜形式粪肠球菌的抗菌活性。根据 ISO6876：2012 测试封闭剂的流动性能。
在整个 30 天实验过程中，BC+1%或 2%DSPs 中的 OCT 释放量在第 2 天超过最低抑菌浓度（MIC）4μg/ml，而 AH+1%或 2%DSPs 中的 OCT 释放量在整个 30 天实验过程中均明显低于 MIC。所有材料（带或不带 DSPs）最初都能杀死浮游细菌。AH±1%或 2%DSPs 在第 7 天无抗菌活性。BC+1%或 2%DSPs 在 30 天内保持抗菌活性。改性和未改性的封闭剂在接触 24 小时后均可完全抑制粪肠球菌生物膜的生长。DSPs 降低了 AH 和 BC 封闭剂的流动性；对于 AH，减少量与添加的 DSPs 量成正比。除 AH+2%DSPs 外，所有改性和未改性的封闭剂均符合 ISO6876 流动测试的可接受标准。
DSPs 增强了 BC 的抗菌性能，但对 AH 没有增强，而材料的流动性能仍符合 ISO6876 标准。根据封闭剂的不同，DSPs 可能会增强根管治疗中的抗菌效果，并可能改善治疗效果。
