Air Force Postgraduate Dental School, Uniformed Services University of the Health Sciences Postgraduate Dental College, Joint Base San Antonio-Lackland, Texas.
Department of Endodontics, University of Texas Health Science Center at San Antonio, San Antonio, Texas.
J Endod. 2019 Jan;45(1):51-56. doi: 10.1016/j.joen.2018.08.007.
Endodontic sealers have traditionally been used to seal dentinal tubules, creating a homogenous interface between the obturation material and the dentinal walls. However, bioceramic sealers have potential added benefits because of their bioactivity. After adequate endodontic therapy, osseous healing is largely dependent on the differentiation and activity of osteoblasts. We hypothesized that EndoSequence BC Sealer (Brasseler, Savannah, GA) and ProRoot ES (Dentsply Tulsa Dental Specialties, Johnson City, TN) have superior biocompatibility and osteogenic potential compared with Roth (Roth International, Chicago, IL) and AH Plus (Dentsply DeTrey Gmbh, Konstanz, Germany) sealers.
A murine osteoblast precursor cell line (IDG-SW3) was exposed to a wide range of concentrations for each of the sealers for 7 days. The relative cell viability was determined by luminescence assay based on adenosine triphosphate quantification (CellTiter-Glo [Promega, Madison, Wisconsin]). The osteogenic potential was determined by fluorescence microscopy of DMP-1 expression, alizarin red staining, and real-time reverse transcription polymerase chain reaction with primers specific for known markers of osteogenesis such as DMP-1, ALP, and Phex. Data were analyzed with 2-way analysis of variance or 1-way analysis of variance with the Bonferroni post hoc test.
Both bioceramic sealers have excellent biocompatibility even at high concentrations. Conversely, cell death was detected when Roth and AH Plus were used at concentrations 100× lower than the bioceramic groups. Importantly, both bioceramic sealers significantly enhanced osteoblastic differentiation although greater responses were noted with EndoSequence BC Sealer. This was evidenced by increased DMP-1 expression, robust up-regulation of osteogenic marker gene expression, and superior mineral deposition. Osteoblastic differentiation and function were significantly impaired when Roth or AH Plus sealer was used.
EndoSequence BC Sealer and ProRoot ES were significantly more biocompatible and promoted osteoblastic differentiation, a bioactivity not found in AH Plus and Roth sealers.
传统上，牙胶根管封闭剂用于封闭牙本质小管，在封闭材料和牙本质壁之间形成同质界面。然而，由于生物陶瓷封闭剂具有生物活性，因此具有潜在的附加益处。在充分的根管治疗后，骨愈合在很大程度上取决于成骨细胞的分化和活性。我们假设 EndoSequence BC 封闭剂（Brasseler，Savannah，GA）和 ProRoot ES（Dentsply Tulsa Dental Specialties，Johnson City，TN）与 Roth（Roth International，Chicago，IL）和 AH Plus（Dentsply DeTrey Gmbh，Konstanz，德国）封闭剂相比，具有更好的生物相容性和成骨潜力。
将鼠成骨前体细胞系（IDG-SW3）暴露于每种封闭剂的多种浓度下 7 天。通过基于三磷酸腺苷定量的发光测定法（CellTiter-Glo [Promega，Madison，Wisconsin]）确定相对细胞活力。通过 DMP-1 表达的荧光显微镜、茜素红染色和针对成骨标志物（如 DMP-1、ALP 和 Phex）的实时逆转录聚合酶链反应确定成骨潜力。使用 2 因素方差分析或 1 因素方差分析，并用 Bonferroni 事后检验对数据进行分析。
即使在高浓度下，两种生物陶瓷封闭剂都具有极好的生物相容性。相反，当 Roth 和 AH Plus 的浓度比生物陶瓷组低 100 倍时，检测到细胞死亡。重要的是，两种生物陶瓷封闭剂都显著增强了成骨细胞分化，而 EndoSequence BC 封闭剂的反应更为明显。这表现为 DMP-1 表达增加、成骨标志物基因表达的强烈上调以及优越的矿化沉积。当使用 Roth 或 AH Plus 封闭剂时，成骨细胞分化和功能显著受损。
EndoSequence BC 封闭剂和 ProRoot ES 明显更具生物相容性，并促进成骨细胞分化，而 AH Plus 和 Roth 封闭剂则没有这种生物活性。
