Driscoll K E, Schlesinger R B
Institute of Environmental Medicine, New York University Medical Center, New York 10016.
Toxicol Appl Pharmacol. 1988 Apr;93(2):312-8. doi: 10.1016/0041-008x(88)90131-7.
The ability of rabbit alveolar macrophages (AM) to release factors which stimulate the migration of peripheral blood neutrophils and monocytes was examined, and the influence of in vitro ozone exposure on this secretory activity was investigated. To evaluate the ability of AM to release leukocyte chemotactic activity, AM obtained by bronchoalveolar lavage were established in monolayer or suspension culture, with and without added zymosan, for 2 and 6 hr. The resulting macrophage-conditioned medium was tested for chemotactic activity using modified Boyden-type chambers and rabbit peripheral blood neutrophils or monocytes as the responding cells. The results demonstrate that substrate attachment (monolayer culture) and/or zymosan phagocytosis can stimulate AM to release chemoattractants for monocytes and neutrophils. Additionally, the results suggest that AM are constitutively producing low levels of monocyte chemotactic factors. The effects of in vitro ozone exposure on the secretion of chemotactic activity was investigated by exposing monolayer cultures of AM to air, 0.1, 0.3, or 1.2 ppm ozone for 2 hr. Macrophage-conditioned medium was harvested immediately, 2 and 6 hr postexposure, and tested for chemotactic activity. Exposure to 0.3 and 1.2 ppm ozone significantly increased the AM secretion of factors which stimulated neutrophil migration; additionally, the results strongly suggest that ozone can augment the ability of AM to stimulate monocyte migration. These results imply a role for the AM in the recruitment of inflammatory cells after ozone inhalation.
研究了兔肺泡巨噬细胞（AM）释放刺激外周血中性粒细胞和单核细胞迁移因子的能力，并调查了体外臭氧暴露对这种分泌活性的影响。为评估AM释放白细胞趋化活性的能力，通过支气管肺泡灌洗获得的AM在单层或悬浮培养中培养，添加或不添加酵母聚糖，培养2小时和6小时。使用改良的博伊登型小室以及兔外周血中性粒细胞或单核细胞作为反应细胞，对所得的巨噬细胞条件培养基进行趋化活性测试。结果表明，底物附着（单层培养）和/或酵母聚糖吞噬作用可刺激AM释放单核细胞和中性粒细胞的趋化因子。此外，结果表明AM持续产生低水平的单核细胞趋化因子。通过将AM单层培养物暴露于空气、0.1、0.3或1.2 ppm臭氧中2小时，研究了体外臭氧暴露对趋化活性分泌的影响。在暴露后立即、2小时和6小时收集巨噬细胞条件培养基，并测试趋化活性。暴露于0.3和1.2 ppm臭氧显著增加了刺激中性粒细胞迁移的因子的AM分泌；此外，结果强烈表明臭氧可增强AM刺激单核细胞迁移的能力。这些结果表明AM在吸入臭氧后炎症细胞的募集过程中发挥作用。
