Isaeva V V, Kerkis A Iu
Tsitologiia. 1988 Jan;30(1):44-8.
A study was made of the ultrastructural reorganization of myosymplasts passed from the primary suspension culture to the secondary monolayer culture. The microtubules in myoblasts and myosymplasts from suspension are very rare, the intermediate filaments form a perinuclear network, small bundles of myofilaments are arranged in disorder, often as whorls around nuclei. After attachment to the solid substrate the myosymplasts form pseudopodia to move as non-muscle fibroblast-like cells. On the leading end of moving symplasts some stress fiber-like structures are found. Numerous microtubules appear. The microtubules and intermediate filaments are arranged in parallel along the axis of a lengthening symplast. A stepwise reorganization of the non-muscle type cytoskeleton to sarcomeres of differentiated myotubes is observed later. The role of attachment and mechanical stress in myotube morphogenesis are discussed.
对从原代悬浮培养传代至二代单层培养的肌质体的超微结构重组进行了研究。悬浮培养的成肌细胞和肌质体中的微管非常稀少，中间丝形成核周网络，小束肌丝排列紊乱，常呈围绕细胞核的螺旋状。附着于固体基质后，肌质体形成伪足，像非肌肉成纤维样细胞一样移动。在移动的肌质体前端发现一些应力纤维样结构。出现大量微管。微管和中间丝沿伸长的肌质体轴平行排列。后来观察到非肌肉型细胞骨架向分化肌管的肌节逐步重组。讨论了附着和机械应力在肌管形态发生中的作用。
