GERM CLEAN 在变形链球菌和白色念珠菌双物种生物膜中的抗龋活性。

Anticaries activity of GERM CLEAN in Streptococcus mutans and Candida albicans dual-species biofilm.

机构信息

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, 610041, China.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Conservation Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

出版信息

Oral Dis. 2022 Apr;28(3):829-839. doi: 10.1111/odi.13799. Epub 2021 Feb 21.

DOI:10.1111/odi.13799

PMID:33583105

Abstract

OBJECTIVE

To evaluate the antimicrobial effects of a peptide containing novel oral spray GERM CLEAN on dual-species biofilm formed by Streptococcus mutans and Candida albicans and to investigate whether GERM CLEAN inhibits the demineralization procedure of bovine enamel in vitro.

METHODS

The antimicrobial effects of GERM CLEAN on dual-species biofilm were analyzed by initial adherence rate calculation, water-insoluble exopolysaccharides quantification, total biomass quantification, and colony-forming units (CFUs) counting. Scanning electron microscopy and confocal laser scanning microscopy were applied to evaluate the impacts of GERM CLEAN on the biofilm structure. Further, the effects of GERM CLEAN on acidogenicity of dual-species were appraised via glycolytic pH drop analysis and hydroxyapatite dissolution measurement. The percentage of Surface Microhardness Reduction (%SMHR) evaluation, Atomic Force Micrograph (AFM) examination, and Transverse Microradiography (TMR) analysis after pH cycling were used to determine whether GERM CLEAN inhibited the demineralization of bovine enamel.

RESULTS

GERM CLEAN decreased the adherence rate, water-insoluble EPS production, biofilm formation, and acidogenicity of the dual-species. Moreover, GERM CLEAN significantly inhibited the demineralization status of bovine enamels.

CONCLUSION

This peptide containing novel oral spray GERM CLEAN has antimicrobial potential toward the dual-species. GERM CLEAN can also impede the demineralization procedure of enamel.

摘要

目的

评估新型口腔喷雾抗菌肽 GERM CLEAN 对变异链球菌和白色念珠菌双物种生物膜的抗菌效果，并研究 GERM CLEAN 是否抑制体外牛牙釉质脱矿过程。

方法

通过初始黏附率计算、水不溶性胞外多糖定量、总生物量定量和菌落形成单位（CFU）计数分析 GERM CLEAN 对双物种生物膜的抗菌效果。扫描电子显微镜和共聚焦激光扫描显微镜用于评估 GERM CLEAN 对生物膜结构的影响。进一步通过糖酵解 pH 值下降分析和羟磷灰石溶解测量评估 GERM CLEAN 对双物种产酸的影响。通过 pH 循环后的表面显微硬度降低率（%SMHR）评估、原子力显微镜（AFM）检查和横向显微放射照相术（TMR）分析来确定 GERM CLEAN 是否抑制牛牙釉质的脱矿。