Nanoscopy and NIC@IIT, Istituto Italiano di Tecnologia, Genoa, Italy.
DIFILAB, Department of Physics, University of Genoa, Genoa, Italy.
J Biophotonics. 2021 Jun;14(6):e202000494. doi: 10.1002/jbio.202000494. Epub 2021 Mar 11.
Many of the most important resolution improvements in optical microscopy techniques are based on the reduction of scattering effects. The main benefit of polarimetry-based imaging to this end is the discrimination between scattering phenomena originating from complex systems and the experimental noise. The determination of the coherency matrix elements from the experimental Mueller matrix can take advantage of scattering measurements to obtain additional information on the structural organization of a sample. We analyze the contrast mechanisms extracted from (a) the coherency matrix elements, (b) its eigenvalues and (c) the indices of polarimetric purity at different stages of zebrafish embryos, based on previous work using Mueller matrix optical scanning microscopy. We show that the use of the coherency matrix and related decompositions leads to an improvement in the imaging contrast, without requiring any complicated algebraic operations or any a priori knowledge of the sample, in contrast to standard polarimetric methods.
许多最重要的光学显微镜技术分辨率的提高都基于散射效应的减少。为此，基于偏振成像的主要优势是区分源自复杂系统的散射现象和实验噪声。从实验 Mueller 矩阵确定相干矩阵元素可以利用散射测量来获取有关样品结构组织的附加信息。我们根据以前使用 Mueller 矩阵光学扫描显微镜的工作，从 (a) 相干矩阵元素、(b) 特征值和 (c) 偏振纯度指数分析了在不同阶段的斑马鱼胚胎中提取的对比机制。我们表明，与标准偏振方法相比，使用相干矩阵及其相关分解可以提高成像对比度，而无需进行任何复杂的代数运算或对样本的任何先验知识。
