Department of Pharmacy, Medical School, Anhui University of Science and Technology, Huainan, P. R. China.
Immunopharmacol Immunotoxicol. 2021 Apr;43(2):223-229. doi: 10.1080/08923973.2021.1885043. Epub 2021 Feb 14.
Puerarin, a natural isoflavone extracted from , is famous for treating various cardiovascular and cerebrovascular diseases. However, little is known about its direct immunomodulatory activity.
This study was designed to investigate the and immunomodulatory effects of by using the murine monocyte-macrophage cell line RAW264.7 and immunosuppressed cyclophosphamide-induced mice.
MTT and neutral red phagocytosis assays were conducted to evaluate the immunomodulatory activities of puerarin on cell viability and phagocytosis by measuring the proliferation, phagocytic, nitric oxide (NO) ability, and TNF-α production ability of stimulated and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Immunosuppressed cyclophosphamide-induced mice were used to evaluate the immunomodulatory activities of puerarin by measuring IL-4 and IFN-γ, the serum half hemolysis value, spleen and thymus index, and proliferation assay for splenic lymphocytes.
Results showed that puerarin improves immunomodulatory activity by increasing cell proliferation, cell phagocytosis, and NO secretion in RAW264.7 macrophages and reduces the abnormal immunologic activity by decreasing cell phagocytosis and NO secretion in LPS-stimulated RAW264.7 macrophages. In addition, puerarin enhanced the immunologic activity of cyclophosphamide-induced immunosuppression mice by increasing the secretion of NO, IFN-γ, and IL-4, the serum half hemolysis value (HC), the spleen and thymus index, and proliferation for splenic lymphocytes.
Puerarin exhibited an upregulated immunomodulatory effect on RAW264.7 macrophages and immunosuppression mice. In addition, puerarin had a downregulated immunomodulatory effect on RAW264.7 macrophages. The results suggest that puerarin could be a promising immunomodulator to assist in the treatment of tumors.
葛根素是从 中提取的天然异黄酮，以治疗各种心脑血管疾病而闻名。然而，其直接免疫调节活性知之甚少。
本研究旨在通过使用小鼠单核巨噬细胞 RAW264.7 细胞系和免疫抑制环磷酰胺诱导的小鼠来研究葛根素的 和 免疫调节作用。
MTT 和中性红吞噬试验用于评估葛根素对细胞活力和吞噬作用的 免疫调节活性，通过测量增殖、吞噬、一氧化氮（NO）能力和刺激和脂多糖（LPS）刺激的 RAW264.7 细胞产生的 TNF-α能力。免疫抑制环磷酰胺诱导的小鼠用于通过测量 IL-4 和 IFN-γ、血清半溶血值、脾和胸腺指数以及脾淋巴细胞增殖试验来评估葛根素的 免疫调节活性。
结果表明，葛根素通过增加 RAW264.7 巨噬细胞的细胞增殖、细胞吞噬和 NO 分泌来改善免疫调节活性，并通过降低 LPS 刺激的 RAW264.7 巨噬细胞的细胞吞噬和 NO 分泌来降低异常免疫活性。此外，葛根素通过增加 NO、IFN-γ和 IL-4 的分泌、血清半溶血值（HC）、脾和胸腺指数以及脾淋巴细胞增殖来增强环磷酰胺诱导的免疫抑制小鼠的免疫活性。
葛根素对 RAW264.7 巨噬细胞和免疫抑制小鼠表现出上调的免疫调节作用。此外，葛根素对 RAW264.7 巨噬细胞表现出下调的免疫调节作用。这些结果表明葛根素可能是一种有前途的免疫调节剂，可用于辅助治疗肿瘤。
