Department of Microbiology, Tokyo Dental College.
Oral Health Science Center, Tokyo Dental College.
Bull Tokyo Dent Coll. 2021 Mar 13;62(1):1-6. doi: 10.2209/tdcpublication.2020-0023. Epub 2021 Feb 15.
Candida albicans is the primary candidiasis-causing fungal pathogen in humans, and one of its most important virulence factors is the ability to form biofilms. Moreover, these biofilms are often resistant to antifungal agents, so there is a need to develop alternative elimination strategies and therapeutic agents for such infections. The antifungal activity of resveratrol, a phytoalexin polyphenolic compound, impairs the morphological transition of C. albicans under various hypha-inducing conditions and inhibits growth of the yeast-form and mycelia. The purpose of this study was to investigate the effect of resveratrol against C. albicans biofilm formation. The developmental, sustained, and mature stages of biofilm formation were affected or inhibited by resveratrol. Exposure to resveratrol at the developmental stage inhibited growth of C. albicans in a dose-dependent manner. A >30% reduction was observed in sustained biofilm growth in the presence of 200 μg/ml resveratrol in comparison with in its absence. In terms of disruption of matured biofilm, 6.25-100 μg/ml resveratrol significantly reduced cell viability of C. albicans compared with in a control sample (p<0.05). The present results indicate that resveratrol has the potential to serve as an anti-Candida treatment and preventive tool which functions by inhibiting existing or under-forming C. albicans biofilms.
白色念珠菌是人类中主要的致病真菌病原体，其最重要的毒力因子之一是形成生物膜的能力。此外，这些生物膜通常对抗真菌药物具有抗性，因此需要开发替代的消除策略和治疗剂来治疗此类感染。白藜芦醇是一种植物抗毒素多酚化合物，具有抗真菌活性，可破坏白色念珠菌在各种诱导菌丝形成条件下的形态转变，并抑制酵母形态和菌丝的生长。本研究旨在研究白藜芦醇对白色念珠菌生物膜形成的影响。白藜芦醇影响或抑制生物膜形成的发育、持续和成熟阶段。在发育阶段暴露于白藜芦醇会以剂量依赖性方式抑制白色念珠菌的生长。与不存在白藜芦醇相比，在存在 200μg/ml 白藜芦醇的情况下，持续生物膜生长减少了>30%。就成熟生物膜的破坏而言，与对照样品相比，6.25-100μg/ml 白藜芦醇显著降低了白色念珠菌的细胞活力(p<0.05)。本研究结果表明，白藜芦醇具有作为抗念珠菌治疗和预防工具的潜力，通过抑制现有的或正在形成的白色念珠菌生物膜发挥作用。
