Huser H, Gerber L, Eichenberger P, Waelti E, Cottier H
Institute of Pathology, University of Bern, Switzerland.
Am J Pathol. 1988 May;131(2):339-43.
The possible role in vivo of osseous structures in binding radioactive iron injected as a low-molecular-weight complex was studied in mice, using combined autoradiography and histomorphometry on sections of undecalcified, plastic-embedded femur epiphyses/metaphyses. A single intraperitoneal injection of 10 microCi 59Fe (1.2 micrograms Fe) per animal as citrate within 3 hours led to a preferential accumulation of this metal in the osteoid mineralized tissue interphase (osteoid seams) of bone. Within the next 2 days the labeling intensity in this localization diminished markedly to approximate levels of the bone marrow and calcified bone. The bulk of the injected radioiron was utilized according to known erythrokinetics. Findings suggest a direct entry of "free," ie, not transferrin-bound, iron into osteoid seams and its consecutive rapid removal from this site.
利用对未脱钙、塑料包埋的股骨骨骺/干骺端切片进行联合放射自显影和组织形态计量学，在小鼠中研究了骨结构在结合作为低分子量复合物注射的放射性铁方面的体内可能作用。在3小时内每只动物腹腔注射10微居里59Fe（1.2微克铁）柠檬酸盐，导致这种金属优先在骨的类骨质矿化组织界面（类骨质缝）中积累。在接下来的2天内，该定位中的标记强度明显降低至接近骨髓和钙化骨的水平。根据已知的红细胞动力学，大部分注射的放射性铁被利用。研究结果表明“游离”（即未与转铁蛋白结合）的铁直接进入类骨质缝，并随后从该部位迅速清除。
