在铁盐存在下超氧化物依赖性生成羟基自由基。通过使用博来霉素依赖性DNA降解检测生物系统中的“游离”铁。
Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of 'free' iron in biological systems by using bleomycin-dependent degradation of DNA.
作者信息
Gutteridge J M, Rowley D A, Halliwell B
出版信息
Biochem J. 1981 Oct 1;199(1):263-5. doi: 10.1042/bj1990263.
Abstract
Bleomycin in the presence of iron(II) degrades DNA to form a thiobarbituric acid-reactive product. This has been made the basis of a specific assay method for 'free' iron in biological fluids. Human synovial fluid, human cerebrospinal fluid and rat pleural-exudate fluid were found to contain micromolar concentrations of 'free' iron, which would be sufficient to allow formation of the hydroxyl radical from superoxide and hydrogen peroxide generated in vivo. This assay method does not detect iron bound to transport proteins or to enzymes.
摘要
博来霉素在亚铁存在的情况下会降解DNA，形成一种能与硫代巴比妥酸反应的产物。这已成为生物体液中“游离”铁的一种特定检测方法的基础。研究发现，人体滑液、人脑脊液和大鼠胸腔渗出液中含有微摩尔浓度的“游离”铁，这足以使体内产生的超氧化物和过氧化氢生成羟基自由基。这种检测方法检测不到与转运蛋白或酶结合的铁。
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