da Cruz Alex Silva, Silva Danilo Conrado, Minasi Lysa Bernardes, de Farias Teixeira Larissa Kamídia, Rodrigues Flávia Melo, da Silva Claudio Carlos, do Carmo Adriana Santana, da Silva Marcos Vinicius Gualberto Barbosa, Utsunomiya Yuri Tani, Garcia José Fernando, da Cruz Aparecido Divino
Mestrado em Genética, Núcleo de Pesquisas Replicon, Escola de Ciências Agrárias e Biológicas, Pontifícia Universidade Católica de Goiás, Goiânia, Brazil.
Curso de Graduação em Medicina Veterinária, Instituto Acadêmico de Ciências Agrárias e Sustentabilidade, Universidade Estadual de Goiás, São Luís de Montes Belos, Brazil.
Front Genet. 2021 Jan 15;11:573344. doi: 10.3389/fgene.2020.573344. eCollection 2020.
Milk production phenotypes are the main focus of genetic selection in dairy herds, and although there are many genes identified as related to the biology of these traits in pure breeds, little is known about crossbreed animals. This study aimed to identify potential genes associated with the 305-day milk yield in 337 crossbreed Gir × Holstein (Girolando) animals. Milk production records were genotyped for 45,613 single-nucleotide polymorphisms (SNPs). This dataset was used for a genome-wide association study (GWAS) using the 305-day milk yield adjusted for the fixed effects of herd and year and linear and quadratic effects of age at calving (in days) and calving factor averaged per animal. Genes within the significant SNPs were retrieved from the ARS-UCD1.2 assembly (bosTau9) for gene ontology analysis. In summary, the GWAS identified 52 SNPs associated [ ≤ 10, false discovery rate (FDR) = 8.77%] with milk production, including NUB1 and SLC24A2, which were previously described as related to milk production traits in cattle. The results suggest that SNPs associated mainly with NUB1 and SLC24A2 could be useful to understand milk production in Girolando and used as predictive markers for selecting genetic predisposition for milk yield in Girolando.
产奶表型是奶牛群遗传选择的主要重点，尽管在纯种中已鉴定出许多与这些性状生物学相关的基因，但对于杂交动物却知之甚少。本研究旨在鉴定337头吉尔×荷斯坦杂交（吉罗兰多）动物中与305天产奶量相关的潜在基因。对45,613个单核苷酸多态性（SNP）进行了产奶记录基因分型。该数据集用于全基因组关联研究（GWAS），使用根据牛群和年份的固定效应以及产犊时年龄（以天为单位）和每头动物平均产犊因子的线性和二次效应调整后的305天产奶量。从ARS-UCD1.2组装（bosTau9）中检索显著SNP内的基因进行基因本体分析。总之，GWAS鉴定出52个与产奶相关的SNP[≤10，错误发现率（FDR）=8.77%]，包括NUB1和SLC24A2，它们先前被描述为与牛的产奶性状相关。结果表明，主要与NUB1和SLC24A2相关的SNP可能有助于了解吉罗兰多牛的产奶情况，并用作选择吉罗兰多牛产奶遗传易感性的预测标记。
