Silvestro L, Viano I, Compagnoni G, Soffiantino M L, Vercellone C, Genazzani E
Institute of Pharmacology, Medical School, University of Torino, Italy.
Anticancer Res. 1988 Jan-Feb;8(1):119-23.
Cells from two leukaemic cell lines (K562 and L1210) were separated on a discontinuous Percoll gradient with densities from 1040 to 1090 by centrifugation. The cells recovered from gradient interfaces were checked for their DNA content and subjected to two different drug sensitivity tests using three cytostatic drugs. The purpose of the study was to compare the sensitivity of a sample of the whole population with that of the fractions thus obtained. Depending on the test used and the line tested, there was a 5.5 to 55.5% discordance between the results for the fractions and the non-separated cells.
将来自两种白血病细胞系（K562和L1210）的细胞通过离心在密度为1040至1090的不连续Percoll梯度上进行分离。检查从梯度界面回收的细胞的DNA含量，并使用三种细胞抑制药物进行两种不同的药物敏感性测试。该研究的目的是比较整个群体样本与由此获得的组分的敏感性。根据所使用的测试和所测试的细胞系，组分与未分离细胞的结果之间存在5.5%至55.5%的不一致。
