枇杷幼果总黄酮的抗多酚氧化酶特性：抑制活性和机制。
Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism.
机构信息
Agricultural Product Storage and Processing Laboratory, Suzhou Academy of Agricultural Sciences , Suzhou, China.
Jiangsu Key Laboratory for Biomass Energy and Material, Institute of Chemical Industry of Forest Products CAF , Nanjing, China.
出版信息
Bioengineered. 2021 Dec;12(1):640-647. doi: 10.1080/21655979.2021.1886387.
This study investigated anti-polyphenol oxidase activity and mechanism of purified total flavonoids (PTF) from young loquat fruits. PTF remarkably inhibited the activity of polyphenol oxidase (PPO) with an value of 21.03 ± 2.37 μg/mL. Based on enzyme kinetics, PTF was found to be a potent, mixed-type, and reversible inhibitor of PPO. The fluorescence intensity of PPO was quenched by PTF through forming a PTF-PPO complex in a static procedure. Therefore, this study authenticated PTF as an efficient PPO inhibitor, which would contribute to their utilization in food industry.
本研究探讨了从枇杷幼果中提取的总黄酮（PTF）的抗多酚氧化酶活性及其机制。PTF 显著抑制多酚氧化酶（PPO）的活性，其半数抑制浓度（IC50）值为 21.03±2.37μg/mL。基于酶动力学研究，发现 PTF 是 PPO 的一种有效、混合型和可逆抑制剂。在静态过程中，PTF 通过形成 PTF-PPO 复合物来猝灭 PPO 的荧光强度。因此，本研究证实 PTF 是一种有效的 PPO 抑制剂，这将有助于其在食品工业中的应用。
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